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Strain Name:
C57BL/6J-MtgxR7916Btlr/Mmmh
Stock Number:
045964-MU
Citation ID:
RRID:MMRRC_045964-MU
Other Names:
R7916 (G1)
Major Collection:

Strain Information

Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, C030040A15Rik, OTTMUSG00000012483
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109241
Homologene: 81861
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77683
Homologene: 11698
Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228829
Homologene: 9507
Gng13
Name: guanine nucleotide binding protein (G protein), gamma 13
Synonyms: Ggamma13
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 64337
Homologene: 81890
Chuk
Name: conserved helix-loop-helix ubiquitous kinase
Synonyms: Chuk1, IKK1, IKK[a], IKK-alpha, IkappaB kinase alpha, IKK 1, IKK alpha, IKKalpha, IKK-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12675
HGNC: HGNC:1974
Homologene: 979
Uhmk1
Name: U2AF homology motif (UHM) kinase 1
Synonyms: KIS, Kist, OTTMUSG00000021542, C820018A03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16589
Homologene: 7801
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,823,086 bp
  • A to G, chromosome 1 at 78,458,563 bp
  • T to A, chromosome 1 at 133,100,904 bp
  • C to T, chromosome 1 at 150,384,498 bp
  • A to G, chromosome 1 at 170,205,188 bp
  • G to T, chromosome 1 at 175,659,670 bp
  • G to A, chromosome 2 at 24,856,696 bp
  • G to A, chromosome 2 at 49,257,106 bp
  • A to C, chromosome 2 at 76,944,521 bp
  • A to T, chromosome 2 at 86,254,858 bp
  • T to A, chromosome 2 at 127,233,059 bp
  • G to A, chromosome 2 at 129,207,837 bp
  • A to G, chromosome 2 at 156,287,938 bp
  • T to C, chromosome 3 at 93,773,279 bp
  • T to A, chromosome 4 at 40,977,142 bp
  • T to C, chromosome 4 at 59,719,841 bp
  • A to G, chromosome 4 at 63,224,552 bp
  • A to C, chromosome 4 at 108,848,246 bp
  • G to T, chromosome 4 at 108,848,247 bp
  • T to C, chromosome 5 at 136,282,961 bp
  • T to TCCG, chromosome 6 at 4,756,451 bp
  • A to T, chromosome 6 at 83,309,473 bp
  • G to A, chromosome 7 at 5,480,819 bp
  • T to C, chromosome 7 at 25,288,290 bp
  • G to A, chromosome 7 at 29,090,939 bp
  • T to G, chromosome 7 at 42,300,511 bp
  • T to G, chromosome 7 at 45,466,115 bp
  • C to A, chromosome 7 at 108,125,122 bp
  • C to T, chromosome 8 at 75,577,950 bp
  • T to A, chromosome 8 at 91,035,056 bp
  • C to T, chromosome 8 at 106,922,865 bp
  • T to G, chromosome 8 at 111,629,391 bp
  • T to C, chromosome 9 at 26,767,424 bp
  • A to T, chromosome 9 at 61,407,128 bp
  • T to C, chromosome 9 at 100,466,251 bp
  • T to C, chromosome 9 at 107,907,578 bp
  • T to G, chromosome 9 at 122,747,434 bp
  • A to T, chromosome 10 at 34,484,025 bp
  • A to G, chromosome 10 at 119,216,588 bp
  • C to A, chromosome 10 at 121,622,943 bp
  • A to T, chromosome 11 at 49,494,716 bp
  • A to G, chromosome 11 at 59,551,863 bp
  • C to A, chromosome 11 at 69,421,148 bp
  • C to A, chromosome 11 at 70,321,285 bp
  • A to G, chromosome 11 at 106,763,862 bp
  • A to G, chromosome 11 at 116,581,058 bp
  • G to T, chromosome 12 at 115,980,989 bp
  • T to C, chromosome 13 at 13,647,072 bp
  • C to A, chromosome 13 at 35,902,051 bp
  • C to T, chromosome 13 at 54,739,450 bp
  • C to T, chromosome 13 at 67,620,175 bp
  • T to A, chromosome 13 at 104,837,473 bp
  • AGAGGAGGAGGAGGAGGAGGA to AGAGGAGGAGGAGGAGGA, chromosome 14 at 21,662,349 bp
  • T to G, chromosome 14 at 43,714,546 bp
  • A to G, chromosome 14 at 52,131,184 bp
  • T to A, chromosome 14 at 66,308,860 bp
  • T to A, chromosome 15 at 77,016,057 bp
  • A to G, chromosome 15 at 78,907,221 bp
  • T to C, chromosome 15 at 81,922,412 bp
  • T to A, chromosome 16 at 4,864,590 bp
  • C to A, chromosome 16 at 10,284,573 bp
  • A to G, chromosome 16 at 94,673,341 bp
  • A to T, chromosome 17 at 25,718,958 bp
  • A to T, chromosome 17 at 46,968,382 bp
  • A to T, chromosome 17 at 81,026,687 bp
  • A to G, chromosome 18 at 37,007,388 bp
  • T to A, chromosome 18 at 37,685,449 bp
  • C to A, chromosome 19 at 4,917,024 bp
  • T to G, chromosome 19 at 9,005,832 bp
  • A to G, chromosome 19 at 44,096,981 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7916 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045964-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.