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Strain Name:
C57BL/6J-MtgxR7921Btlr/Mmmh
Stock Number:
045968-MU
Citation ID:
RRID:MMRRC_045968-MU
Other Names:
R7921 (G1)
Major Collection:

Strain Information

Hdgfl2
Name: HDGF like 2
Synonyms: HRP-2, Hdgfrp2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15193
VEGA: 17
Homologene: 7355
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Nucks1
Name: nuclear casein kinase and cyclin-dependent kinase substrate 1
Synonyms: Nucks, 2700010L10Rik, 8430423A01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98415
Homologene: 23377
Fbxo22
Name: F-box protein 22
Synonyms: 1600016C16Rik, 0610033L19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71999
Homologene: 12433
Fgr
Name: FGR proto-oncogene, Src family tyrosine kinase
Synonyms: Ali18, Mhdaali18
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14191
HGNC: HGNC:3697
Homologene: 3842
Fkbp9
Name: FK506 binding protein 9
Synonyms: FKBP60, FKBP63
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 27055
HGNC: HGNC:3725
Homologene: 31434
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 87,924,084 bp
  • A to G, chromosome 1 at 93,012,008 bp
  • G to C, chromosome 1 at 93,567,515 bp
  • G to T, chromosome 1 at 131,910,736 bp
  • G to A, chromosome 1 at 139,062,873 bp
  • A to T, chromosome 1 at 195,151,667 bp
  • G to A, chromosome 2 at 13,424,727 bp
  • G to A, chromosome 2 at 23,545,478 bp
  • A to G, chromosome 2 at 68,307,039 bp
  • A to G, chromosome 2 at 73,310,455 bp
  • A to G, chromosome 2 at 76,585,097 bp
  • A to T, chromosome 2 at 90,998,747 bp
  • A to G, chromosome 2 at 91,548,940 bp
  • G to T, chromosome 2 at 92,953,646 bp
  • C to A, chromosome 2 at 119,530,099 bp
  • T to A, chromosome 2 at 119,919,678 bp
  • T to G, chromosome 3 at 95,326,399 bp
  • A to T, chromosome 3 at 138,224,010 bp
  • G to T, chromosome 3 at 145,474,238 bp
  • T to G, chromosome 4 at 132,986,521 bp
  • A to G, chromosome 4 at 135,929,910 bp
  • T to C, chromosome 4 at 143,656,565 bp
  • A to T, chromosome 4 at 152,028,661 bp
  • T to G, chromosome 4 at 152,303,158 bp
  • A to C, chromosome 5 at 14,669,234 bp
  • C to T, chromosome 5 at 30,970,150 bp
  • A to G, chromosome 5 at 88,554,896 bp
  • A to G, chromosome 5 at 100,061,133 bp
  • T to A, chromosome 5 at 112,335,576 bp
  • A to T, chromosome 5 at 119,680,869 bp
  • G to T, chromosome 5 at 119,680,870 bp
  • G to T, chromosome 5 at 143,174,262 bp
  • G to A, chromosome 5 at 145,791,688 bp
  • A to G, chromosome 6 at 29,447,770 bp
  • A to G, chromosome 6 at 56,851,385 bp
  • C to T, chromosome 6 at 90,667,941 bp
  • T to C, chromosome 6 at 118,465,071 bp
  • G to A, chromosome 6 at 119,317,367 bp
  • T to G, chromosome 6 at 121,677,995 bp
  • T to C, chromosome 7 at 55,979,810 bp
  • A to G, chromosome 7 at 101,250,542 bp
  • A to T, chromosome 7 at 103,122,428 bp
  • A to T, chromosome 7 at 120,182,769 bp
  • A to T, chromosome 7 at 135,695,204 bp
  • A to T, chromosome 7 at 140,202,299 bp
  • G to C, chromosome 7 at 141,809,687 bp
  • T to A, chromosome 8 at 4,515,704 bp
  • T to C, chromosome 8 at 91,042,281 bp
  • T to A, chromosome 8 at 94,487,664 bp
  • C to A, chromosome 8 at 105,691,104 bp
  • T to A, chromosome 8 at 125,837,863 bp
  • T to A, chromosome 9 at 18,659,318 bp
  • A to T, chromosome 9 at 24,422,338 bp
  • C to T, chromosome 9 at 26,773,968 bp
  • A to T, chromosome 9 at 55,218,353 bp
  • A to G, chromosome 9 at 105,414,687 bp
  • A to T, chromosome 10 at 78,073,740 bp
  • G to A, chromosome 10 at 78,073,952 bp
  • A to T, chromosome 10 at 80,530,603 bp
  • T to C, chromosome 10 at 81,390,992 bp
  • T to G, chromosome 10 at 128,043,049 bp
  • T to C, chromosome 11 at 58,801,781 bp
  • T to C, chromosome 11 at 67,283,818 bp
  • T to A, chromosome 11 at 69,100,779 bp
  • T to A, chromosome 11 at 69,520,834 bp
  • C to T, chromosome 11 at 84,908,238 bp
  • A to T, chromosome 11 at 115,887,178 bp
  • A to T, chromosome 11 at 116,297,682 bp
  • G to A, chromosome 13 at 34,747,101 bp
  • T to A, chromosome 13 at 67,256,116 bp
  • C to A, chromosome 13 at 96,424,780 bp
  • A to T, chromosome 13 at 112,601,507 bp
  • T to C, chromosome 14 at 7,933,800 bp
  • T to C, chromosome 14 at 20,304,072 bp
  • A to G, chromosome 14 at 93,015,565 bp
  • C to A, chromosome 15 at 5,177,151 bp
  • A to T, chromosome 15 at 18,991,956 bp
  • A to T, chromosome 15 at 66,683,793 bp
  • A to T, chromosome 15 at 71,981,962 bp
  • A to T, chromosome 15 at 89,306,223 bp
  • T to A, chromosome 15 at 100,353,370 bp
  • G to A, chromosome 16 at 32,751,321 bp
  • GCCCAAAC to G, chromosome 16 at 46,038,480 bp
  • T to C, chromosome 17 at 6,905,091 bp
  • A to T, chromosome 17 at 20,113,405 bp
  • C to T, chromosome 17 at 37,395,442 bp
  • T to C, chromosome 17 at 56,093,724 bp
  • C to T, chromosome 18 at 32,123,417 bp
  • T to C, chromosome 18 at 37,478,245 bp
  • A to T, chromosome 18 at 65,725,383 bp
  • A to G, chromosome 18 at 84,622,258 bp
  • T to C, chromosome 19 at 4,271,030 bp
  • C to G, chromosome 19 at 4,749,012 bp
  • T to A, chromosome 19 at 38,620,553 bp
  • G to A, chromosome 19 at 44,235,892 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7921 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045968-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.