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Strain Name:
B6;129S-Ugdhtm1.1Xzh/Mmjax
Stock Number:
046262-JAX
Citation ID:
RRID:MMRRC_046262-JAX
Other Names:
Ugdh Flox

Strain Information

Ugdhtm1.1Xzh
Name: UDP-glucose dehydrogenase; targeted mutation 1.1, Xin Zhang
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Conditional
Ugdh
Name: UDP-glucose dehydrogenase
Synonyms: Udpgdh
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Conditional
NCBI: 22235
Homologene: 2520
Genetic Alterations
An FRT-flanked neo cassette and loxP site were inserted upstream of Ugdh exon 6 and a loxP site was inserted downstream of exon 6 via homologous recombination. Flp-mediated recombination removed the neo cassette.
Genotype Determination
ES Cell Line
Not specified
Phenotype
None/Normal/Wild-type
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Crosses, Genetic
  • Diffusion
  • Fibroblast Growth Factor 10/metabolism
  • Fibroblast Growth Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Genomics
  • Glycosaminoglycans/metabolism
  • Heparitin Sulfate/metabolism
  • Lacrimal Apparatus/metabolism
  • Ligands
  • Mice
  • Mice, Inbred C57BL
  • Models, Biological
  • Models, Genetic
  • Signal Transduction
  • Uridine Diphosphate Glucose Dehydrogenase/metabolism
Strain Development
The targeting vector was constructed using the recombineering method from a 12.7-kb genomic fragment, which was retrieved from a C57BL/6 BAC clone (RP23-477N9, BACPAC Resources Center at Children's Hospital Oakland Research Institute). It contains a neomycin (Neo) selection cassette surrounded by two FRT sites and exon 6 of the Ugdh gene flanked by two loxP sites. The linearized targeting construct was electroporated into an ES cell line of 129S6/SvEvTac origin. Recombinant clones were screened by Southern blot analysis with 5’ (SacI) and 3’ (BamHI) external probes before being injected into C57BL/6 blastocysts. Chimeras were further bred with C57BL/6 mice for germline transmission, which was confirmed by PCR genotyping using the following primers: UgdhfloxF: 5’-TTCTGAGGCTGTATTTCACTTCC-3’; UgdhfloxR: 5’-AGGCACAGGCACGATTAGGA-3’. Amplification bands of 214 bp and 314 bp correspond to the wild-type and floxed alleles, respectively. The FRT-flanked Neo cassette was removed by crossing with an FLP-transgenic line (B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ - JAX Strain #009086).
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Developmental Biology
Donor
Xin Zhang, Ph.D Columbia University Medical Center
Primary Reference

Qu X, Pan Y, Carbe C, Powers A, Grobe K, Zhang X. Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development.Development. 2012 Aug;139(15):2730-9. doi: 10.1242/dev.079236. Epub 2012 Jun 28. PubMed PMID: 22745308; PubMed Central (Medline PMCID: PMC3392702)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
046262-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
046262-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.