Strain Name:
B6J(Cg)-Cd22tm2.1Tft/Mmnc
Stock Number:
046263-UNC
Citation ID:
RRID:MMRRC_046263-UNC
Other Names:
CD22AA, B6J.Cg-Cd22tm2Tft/Mmnc

Strain Information

Cd22tm2Tft
Name: CD22 antigen; targeted mutation 2, Thomas F Tedder
Synonyms: CD22AA
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knock-In
Cd22
Name: CD22 antigen
Synonyms: Lyb-8, Lyb8
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knock-In
NCBI: 12483
HGNC: HGNC:1643
Homologene: 31052
Genetic Alterations
CD22 that generates a full-length CD22 containing point mutations of R130 and R137 to uncharged alanine residues (CD22AA)

HGVS nomenclature:
  • mRNA RefSeq: NM_001043317.2
  • protein RefSeq: NP_001036782.1
  • nucleic acid level alteration: c.388A>/c.389G>C and c.409C>G/c.410G>C (this assumes the simplest possible changes)
  • predicted amino acid level alteration: p.Arg130Ala and p.Arg137Ala
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    R1
    Phenotype
    CD22 expression was reduced by 20-44%. Demonstrated reduction in surface IgM expression and in recirculating bone marrow B cells (59%), marginal zone B cells (68%). BCR-induced proliferation by CD22AA B cells after 72 h in culture was considerably reduced over a range of F(ab′)2 anti-IgM concentrations compared with that of wild-type B cells, as evaluated by [3H]thymidine uptake.
    Mammalian Phenotype Terms
    Allelic Composition: (Genetic Background: )

    MeSH Terms
    • Animals
    • Antigens, CD/physiology
    • Antigens, Differentiation, B-Lymphocyte/physiology
    • B-Lymphocytes/physiology
    • Cell Adhesion Molecules/physiology
    • Cell Movement
    • Lectins/physiology
    • Ligands
    • Lymphocyte Activation
    • Mice
    • N-Acetylneuraminic Acid/metabolism
    • Receptors, Antigen, B-Cell/physiology
    • Sialic Acid Binding Ig-like Lectin 2
    Strain Development
    R1 embryonic stem cells were electroporated with the linearized DNA and selected for G418 resistance. Correctly targeted embryonic stem cell clones were identified by Southern blot analysis of genomic DNA and were injected into 3.5-day-old B6 blastocysts before being transferred into pseudopregnant female mice. Chimeric offspring from one embryonic stem clone were bred with B6J mice (JAX Strain #00064) to provide germline transmission of the disrupted allele, generating CD22AAneo heterozygous offspring. Heterozygous CD22AAneo mice were then crossed with B6.C-Tg(CMV-cre)1Cgn/J (JAX Strain #006054) to facilitate removal of neor, which was confirmed by Southern blot analysis of DNA obtained from tail tissue. Heterozygous offspring on a mixed B6/129 genetic background were interbred to generate homozygous CD22AA and wild-type littermates lacking both neor and the gene encoding Cre. The deposited animals were backcrossed to B6J for more than 6 generations.
    Suggested Control Mice
    C57BL/6
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cell Biology
    • Hematology
    • Immunology and Inflammation
    • Models for Human Disease
    • Research Tools
    Donor
    John Poe, Ph.D Duke University Medical Center
    Primary Reference

    Poe JC, Fujimoto Y, Hasegawa M, Haas KM, Miller AS, Sanford IG, Bock CB,Fujimoto M, Tedder TF. CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms. Nat Immunol. 2004Oct;5(10):1078-87. Epub 2004 Sep 19. (Medline PMID: 15378059)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Generation
    N6+F6+
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 8 to 12 months 8 to 12 months
    Average litter size
    7-9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    5-9

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    046263-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.