Strain Detail Sheet

Strain Name:
B6.129-Tg(ITGAL-Ptprca)LP8Rasch Ptprctm1Holm/Mmucd
Stock Number:
046267-UCD
Citation ID:
RRID:MMRRC_046267-UCD
Other Names:
CD45 minigene transgenic strain LP8

Strain Information

Gene Details

Ptprctm1Holm
Name: protein tyrosine phosphatase receptor type C; targeted mutation 1, Nick Holmes
Synonyms: Ptprctm1, CD45-, CD45-exon9
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Ptprc
Name: protein tyrosine phosphatase receptor type C
Synonyms: T200, B220, CD45, Lyt-4, Ly-5
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Genome Browser [Click image to go to Jbrowse]



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Ptprca
Name: protein tyrosine phosphatase receptor type C; a variant
Synonyms: PtprcSJL, Ly5a, CD45.1
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Transgenic
Tg(ITGAL-Ptprca)LP8Rasch
Name: transgene insertion LP8, William C Raschke
Type: Allele
Species: Multi-species
Chromosome: unknown
Alteration at locus: Transgenic
ITGAL
Name: integrin subunit alpha L
Type: Regulatory Region
Species: Homo sapiens (human)
Chromosome: 16
Alteration at locus: Transgenic
Genetic Alterations
CD45-minigene transgenic strain LP8 on the CD45 knockout C57BL/6 background (JAX Strain #002725) expresses 63% of the wild-type level of CD45.
Genotype Determination
Genotyping Protocol
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+

Strain Description

Phenotype
CD45-minigene transgenic strain LP8 on the CD45 knockout C57BL/6 background (JAX Strain #002725) expresses 63% of the wild-type level of CD45. This level is the same as mice with one functional copy of the wild-type endogenous allele (mice heterozygous for the knockout allele) and also the same as expressed by CD45-minigene transgenic F (MMRRC:46054). The transgene encodes the CD45.1 allelic sequence which is detected by anti-CD45.1 antibodies. This strain, like all of the CD45-minigene transgenics, expresses normal cell lineage-specific isoform regulation. The strain has no breeding, lifespan or general health deficiencies.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
Strain Development
The CD45-minigene transgenic construct 'VGI-1084' was injected into C57BL/6J zygotes, and 7 founders were identified: LP4, LP5H, LP5L, LP8, LP10, LP26, and LP28. VGI-1084 contains the same CD45 minigene construct as VGI-0020 (also known as pLFAiT200 in PMID:12393487) but has a LoxP site added at each end of the minigene. The LoxP sites allow the minigene to be deleted from the genome in the presence of cre. CD45-minigene transgenic LP8 (the ‘LP’ series designating the presence of the LoxP sites) was one of the founders. The LP8 founder was mated to C57BL/6J mice and progeny were screened for the presence of the transgene. The colony was then crossed onto the CD45-null background by matings with CD45 knockout mice also on the C57BL/6 background (JAX Strain #002725). LP8 mice homozygous for the transgene locus and on the CD45 knockout background were then sibling mated for at least 10 generations to date. The LP8 mice express functional CD45 protein solely from the CD45 minigene.

Note: This strain is part of a family of CD45 minigene strains, which includes MMRRC:44051, 46054, 46256, 46257, 46258, 46266, 46267, 46268, 46269, and 46270.
Suggested Control Mice
C57BL/6J (JAX Strain #000664) and CD45 knockout on C57BL/6 (JAX Strain #002725)

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease

Strain Origin

Donor
William Raschke, Ph.D Virogenics

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black
Eye
Black
Other
CD45-minigene transgenic LP8 is one of a series of transgenic strains prepared with the CD45 minigene. Strain LP8 expresses 63% of the wild-type level of CD45, which is equivalent to the level expressed by mice with one functional copy of the wild-type endogenous allele and by CD45-minigene transgenic F. Both the heterozygous CD45 knockout and transgenic strain F mice were instrumental for establishing that CD45 reduction provides protection from lethal effects of multiple pathogens, as these strains are resistant to lethal doses of B. anthracis, Ebola, and other pathogens (published), unlike wild-type mice and strains expressing low levels of CD45. CD45-minigene transgenic strain LP8 offers a substantial benefit by having the added feature of the minigene being flanked by LoxP sequences, such that crosses of LP8 mice with cre-expressing strains result in transgene excision and 0% CD45 expression in cells expressing cre. Crosses of strain LP8 with vav-icre mice (JAX Strain #008610) produce mice with 0% expression of CD45 in hematopoietic cells in which vav is expressed. Strain LP8 is therefore suitable for generating conditional mutants lacking CD45 expression in specific hematopoietic subpopulations using the many strains available with cell type-specific cre expression. This strain should be particularly suitable for studies on the cell types responsible for the disease protection reported in mice with reduced CD45 expression. Strain LP8 is also a replacement for transgenic strain F (MMRRC:46054) for studies of the effect of CD45 expression level, and thus CD45 phosphatase activity, on lymphocyte activation and maturation. This strain should be also suitable for similar activation and maturation studies of other hematopoietic lineages which express CD45, especially using the LoxP feature with conditional cre expression.

Breeding

MMRRC Breeding System
Sib-mating
Generation
F10
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
7-9
Recommended wean age
4 Weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
046267-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit		 Aliquot Approximate quantity3
046267-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.