Strain Name:
B6N.Cg-Ifitm5tm1Pmof/Mmjax
Stock Number:
046274-JAX
Citation ID:
RRID:MMRRC_046274-JAX
Other Names:
Bril-KO

Strain Information

Ifitm5tm1Pmof
Name: interferon induced transmembrane protein 5; targeted mutation 1, Pierre Moffatt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Ifitm5
Name: interferon induced transmembrane protein 5
Synonyms: Hrmp1, fragilis4, 1110003J06Rik, Bril
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 73835
Homologene: 14210
Genetic Alterations
Replacement of the two coding exons of the Ifitm5 gene.
Genotype Determination
ES Cell Line
R1
Phenotype
None/Normal/Wild-type
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Bone Development/genetics
  • Bone Development/physiology
  • Calcium/metabolism
  • Female
  • Homeostasis/genetics
  • Homeostasis/physiology
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Knockout
  • Osteoblasts/cytology
  • Osteoblasts/metabolism
  • Osteogenesis/genetics
  • Osteogenesis/physiology
  • Osteogenesis Imperfecta/genetics
  • Osteogenesis Imperfecta/metabolism
  • Pregnancy
  • Protein Binding
  • Reverse Transcriptase Polymerase Chain Reaction
Strain Development
A targeting vector was constructed to create a global conventional gene knockout for Ifitm5 (Bril). The targeting strategy replaced the two coding exons of the Ifitm5 gene by the nuclear localization signal (NLS)-tagged LacZ (NLS-LacZ) and a LoxP-pGK-Neo-LoxP cassette configured in a tail-to-tail orientation. The two homology arms consisted of a 3.9-kb fragment upstream of the natural translation start site (iMET), and a 5-kb fragment covering the intergenic region downstream of exon 2. The targeting vector was linearized with NotI and electroporated into R1 ES cells. G418-resistant clones were screened by Southern blotting for correct targeting of the Ifitm5 locus. Positive ES clones were selected, expanded, and injected into blastocysts that were implanted into pseudo-pregnant C57BL6 females to generate chimeric mice. Chimeras were bred to wild-type C57BL/6N and descendants genotyped by PCR on gDNA isolated from tail clips. Mice have been backcrossed for 7 generations onto C57BL/6N (Charles River Laboratory Strain #027).
Suggested Control Mice
C57BL/6N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Internal/Organ
  • Research Tools
Donor
Pierre Moffatt, Ph.D Shriners Hospitals for Children - Canada
Primary Reference
Patoine A, Husseini A, Kasaai B, Gaumond MH, Moffatt P. The osteogenic cellsurface marker BRIL/IFITM5 is dispensable for bone development and homeostasis inmice. PLoS One. 2017 Sep 7;12(9):e0184568. doi: 10.1371/journal.pone.0184568.eCollection 2017.(Medline PMID:28880886)

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N = 8 (C57BL/6N)
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined 6 to 8 months
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
046274-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
046274-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.