Strain Detail Sheet

Strain Name:
C57BL/6J-Del(XMageb5)1Jbmu/Mmnc
Stock Number:
046276-UNC
Citation ID:
RRID:MMRRC_046276-UNC
Other Names:
Del(XMageb5)1Jbmu

Strain Information

Gene Details

Del(XMageb5)1Jbmu
Name: deletion, chr X, Jacob Mueller 1; deletion, chr X, Jacob Mueller 1
Synonyms: Mageb5DelArm1, Del1Jbmu
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Deletion
Mageb5
Name: MAGE family member B5
Synonyms: mMage-b5, 1700056A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Deletion
NCBI: 74271
Homologene: 135943
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Genetic Alterations
Deletion of one of two palindrome arms, each of which harbors a copy of Mageb5.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.

    • Strain Description

    Phenotype
    Expression levels of Mageb5 in the single palindrome arm deletion mice are approximately half that of wild-type mice. This strain would be useful for making a Mageb5 null mouse, as one palindrome arm has already been deleted.
    Strain Development
    To generate mice carrying palindrome arm deletions, the donor used CRISPR with dual single-guide RNAs (sgRNAs), designed to unique sequences flanking the targeted palindrome arm, as close to the edge of the arm as possible. After active sgRNAs were identified for both sides of each targeted palindrome arm, two pX458M plasmids encoding the sgRNAs and Cas9 were injected into the pronucleus of (C57BL/6JXSJL)F1 mouse zygotes. The zygotes were generated from mating C57BL/6J females to (C57BL/6JXSJL)F1 males so that all targeted X chromosomes were C57BL/6J. Blastocysts from the pronuclear injection were implanted into pseudopregnant females. Genomic DNA from resulting pups was screened by PCR and Sanger sequencing of the deletion junctions. Male and female mice were able to transmit the Mageb5 deletion through the germline.
    Suggested Control Mice
    wild-type littermates

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on two XY samples VL3212 and EI5170 at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and VL3212 is the representative sample selected for the report. The background is C57Bl/6J and there is no evidence of a secondary background. The mitochondrial genome and Y chromosome are consistent with the C57BL/6J background and none of the constructs detectable by MiniMUGA are present.

    The genome of the MMRRC: 46276 strain is 100 % inbred.
    MiniMUGA Sample Report
    MiniMUGA Sample Report Link

    Research Applications

    • Reproduction

    Strain Origin

    Donor
    Jacob Mueller, Ph.D University of Michigan Medical School
    Primary Reference
    Kruger AN, Ellison Q, Brogley MA, Gerlinger ER, Mueller JL. Male mice with large inversions or deletions of X-chromosome palindrome arms are fertile and express their associated genes during post-meiosis. Sci Rep. 2018 Jun 12;8(1):8985. doi: 10.1038/s41598-018-27360-x. (Medline PMID:29895860)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    N10 (C57BL/6J)
    Overall Breeding Performance
    Good

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes X-linked
    Homozygotes are fertile: Yes X-linked
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4-6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    046276-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.