Strain Name:
STOCK Ssrp1tm1c(EUCOMM)Wtsi/GukMmnc
Stock Number:
046323-UNC
Citation ID:
RRID:MMRRC_046323-UNC
Other Names:
B6.129s Ssrp1fl/fl

Strain Information

Ssrp1tm1.1Guk
Name: structure specific recognition protein 1; targeted mutation 1.1, Katerina Gurova
Synonyms: Ssrp1fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Conditional
Ssrp1
Name: structure specific recognition protein 1
Synonyms: Hmgox, Hmgi-rs3, Hmg1-rs1, T160
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Conditional
NCBI: 20833
Homologene: 110735
Genetic Alterations
Conditional knockout of the Ssrp1 gene: exons 4-9 are floxed.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    G4 derived from (129S6/SvEvTac x C57BL/6Ncr)F1
    Phenotype
    None/Normal/Wild-type
    Strain Development
    The Ssrp1 targeting vector was obtained from the European Mutant Mouse Consortium (Ssrp1tm1a(EUCOMM)Wtsi - MGI:4461716, targeting vector PG00202_Z_1_D01) to create a conditional knockout of the Ssrp1 gene. The vector was electroporated into G4 embryonic stem cells (PMID:17360545) by the Gene Targeting and Transgenic Shared Resource at Roswell Park Comprehensive Cancer Center. Clones of the cells with an integrated insert were selected using G418. Clones positive for homologous recombination were identified via Southern blotting and then expanded for injection into C57BL/6J albino host embryos. Embryos were transferred into CD-1 pseudopregnant foster mice. Chimeras were generated from three ES cell clones, and germline transmission was achieved. Chimera mice (Ssrp1Neo LacZ fl/+) containing the targeting vector were bred with B6albinoJ (Ssrp1+/+) wild-type mice to generate heterozygous F1 progeny (Ssrp1Neo LacZ fl/+). Ssrp1Neo LacZ fl/+ were crossed with B6;129S4-Gt(ROSA)26Sortm1Sor/J (JAX Strain #003309) mice to excise the synthetic cassette (FRT Neo LacZ) to generate heterozygous (Ssrp1fl/+) mice. Ssrp1fl/+ were bred within the same genotype to generate homozygous (Ssrp1fl/fl) mice. The line was archived using C57BL/6J (JAX Strain #000664) egg donors.
    Suggested Control Mice
    wild-type C57BL/6J
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    Donor
    Katerina Gurova, Ph.D Roswell Park Comprehensive Cancer Center
    Primary Reference

    Sandlesh P, Juang T, Safina A, Higgins MJ, Gurova KV. Uncovering the fine print of the CreERT2-LoxP system while generating a conditional knockout mouse model of Ssrp1 gene. PLoS One. 2018 Jun 28;13(6):e0199785. doi: 10.1371/journal.pone.0199785. (Medline PMID: 29953487)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Sib-mating
    Generation
    N/A
    Overall Breeding Performance
    Excellent
    Bred to Homozygosity
    Yes
    Average litter size
    4-6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    046323-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.