Strain Name:
C57BL/6N-Tg(Dmp1-Gja1,-GFP*)82Plot/Mmmh
Stock Number:
046324-MU
Citation ID:
RRID:MMRRC_046324-MU
Other Names:
DMP1-8kb-Cx43

Strain Information

Tg(Dmp1-Gja1,-GFP*)82Plot
Name: transgene insertion 82, Lilian Plotkin
Type: Allele
Species: Multi-species
Chromosome: unknown
GFP
Name: green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Dmp1
Name: dentin matrix protein 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13406
HGNC: HGNC:2932
Homologene: 68396
Gja1
Name: gap junction protein, alpha 1
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 20
Genetic Alterations
DMP1-8kb-Cx43/GFP mice (abbreviated as Cx43 OT) were generated at the Transgenic and KO Mouse Core (IUSM) with the Cx43 transgene, generated by S and M Harris (UTSHC, San Antonio, TX, USA), using the DMP1-8kb promoter CMV-Cx43/ GFP constructs. Mouse DMP-1 cis-regulatory sequences containing 7892 bp of the promoter region and a 4439-bp region containing the first exon, the entire first intron, and a small part of Exon 2 were used. This DNA fragment was cloned into a vector (pBS-SK) that contained the topaz variant of green fluorescent protein (GFP-tpz as used in PMID:15207743). Rat Cx43 cDNA in Bluescript M13+ vector was amplified by oligonucleotides 5'-CCCTCGAGGGAAGGCGTGAGGAAAGTAC-3' (RAT43F) and 5'-CGGGATCCAAGCCGGTTTAAATCTCCAG-3' (RAT43R), which added a XhoI restriction site at the 5' end and a BamHI restriction site at the 3' end of the cDNA. The amplified Cx43 cDNA was subcloned into pGEM-T Easy vector system I (Promega, Madison, WI).
Genotype Determination
Phenotype
The over-expression of Cx43 in osteocytes using the DMP1-8kb promoter (Cx43OT) does not have any overall visible changes. However, it reduces age-associated bone changes by maintaining osteocyte viability and bone formation, thus leading to improved bone strength.
Strain Development
The transgenic construct DMP-Cx43 was introduced by pronuclear injection. 11 C57BL/6Nhsd founders were identified. Of those, 2 were found to express the transgene and to transmit it to the progeny. Line #82 was selected for further breeding and expansion. Mice were then sibling mated for at least 5 generations to date.
Suggested Control Mice
C57BL/6Nhsd
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Research Tools
Donor
Lilian Plotkin, Ph.D IU School of Medicine
Primary Reference

Davis HM, Aref MW, Aguilar-Perez A, Pacheco-Costa R, Allen K, Valdez S,Herrera C, Atkinson EG, Mohammad A, Lopez D, Harris MA, Harris SE, Allen M,Bellido T, Plotkin LI. Cx43 overexpression in osteocytes prevents osteocyteapoptosis and preserves cortical bone quality in aging mice. JBMR Plus. 2018Jul;2(4):206-216. doi: 10.1002/jbm4.10035. Epub 2018 Jan 18. (Medline PMID: 29978155)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
F5
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 6 to 8 months
Average litter size
4-6
Recommended wean age
3 Weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
046324-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
046324-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.