Strain Name:
B6J.129-SgceGt(148G1)Cmhd/Mmmh
Stock Number:
050517-MU
Citation ID:
RRID:MMRRC_050517-MU
Other Names:
Homozygotes (SgceGt(148G1)Cmhd/Gt(148G1)Cmhd or SgceGt/Gt)

Strain Information

SgceGt(148G1)Cmhd
Name: sarcoglycan, epsilon; gene trap 148G1, Centre for Modeling Human Disease
Synonyms: SgceGt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
Sgce
Name: sarcoglycan, epsilon
Synonyms: e-SG
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Gene Trap
NCBI: 20392
Homologene: 31205
Genetic Alterations
Gene-trapped ES cells (CMHD-GT_148G1-3, intron 9 of NM_011360) were used to generate a novel Sgce mouse model on C57BL/6J background with markedly reduced expression of isoforms derived from exons 3' to exon 9 of NM_011360.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Sgcem+/pGt mice exhibited “tiptoe” gait and stimulus-induced appendicular posturing between P14 and P16. Abnormalities noted in older Sgcem+/pGt mice included reduced body weight, altered gait dynamics, and reduced open-field activity
Strain Development
R1 embryonic stem cells and the Gep-SD5 vector (clone number CW509161 [CMHD-GT_148G1-3]) was obtained from The Center for Modeling Human Disease (CMHD, Ontario, Canada; www.cmhd.ca/genetrap/vectors.html). The Gep-SD5 vector does not contain an internal ribosome entry sequence. Due to the rules of nonsense-mediated decay, the selection transcript is only produced if the insertion site is close to the polyA near the 3′ end of the gene. The polyA sequence of the reporter gene (lacZ) is deleted which results in an unstable trapped transcript leading to hypomorphic mutants. DNA was extracted from cultured ES cells and used to confirm the correct targeting of Sgce. Gene-trapped ES cells were injected into the blastocoel cavity of E3.5 C57BL/6 embryos using standard procedures. Male chimeras were bred to C57BL/6J female mice to establish coat color and germline transmission. The insertion site of the gene trap was identified with long-range PCR and confirmed with Sanger sequencing. We established that the terminal exons (3′ to exon 9) of Sgce were disrupted in homozygotes (SgceGt(148G1)Cmhd/Gt(148G1)Cmhd or SgceGt/Gt) and paternal heterozygotes (Sgcem+/pGt, m-maternal, p-paternal). Male Sgcem+/pGt mice were crossed to C57BL/6J female mice for 10 generations
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Mark Ledoux, Ph.D University of Tennessee Health Science Center

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N10+F5+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
4-6
Recommended wean age
4 Weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050517-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
050517-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.