Strain Name:
B6J.129P2(Cg)-Cacna1etm1.1Tsch/Mmjax
Stock Number:
050523-JAX
Citation ID:
RRID:MMRRC_050523-JAX
Other Names:
Ta1E-|-

Strain Information

Cacna1etm1.1Tsch
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit; targeted mutation 1.1, Toni Schneider
Synonyms: Cav2.3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: alpha1E, Cchra1, Cav2.3
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Genetic Alterations
Ubiquitous knockout of the Cacna1e gene by deletion of exon 2.
Genotype Determination
ES Cell Line
E14.1 derived from 129P2/OlaHsd
Phenotype
Animals deficient for Cav2.3 show a mild phenotype: they live as long as control mice with the identical genetic background, but they present with some neuronal, endocrine, and cardiac deficiencies, such as susceptibility to convulsive drugs, increased anxiety & aggression, cardiac arrhythmia, and altered peptide hormone secretion (e.g., insulin, glucagon, and somatostatin).
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Calcium Channels/genetics
  • Calcium Channels/physiology
  • Calcium Channels, R-Type
  • Cation Transport Proteins
  • Female
  • Glucose/physiology
  • Glucose Tolerance Test
  • Homeostasis
  • Hyperglycemia/etiology
  • Injections, Intraperitoneal
  • Insulin/metabolism
  • Islets of Langerhans/cytology
  • Islets of Langerhans/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Phenotype
  • Stress, Physiological/complications
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cardiovascular
  • Diabetes
  • Neurobiology
  • Reproduction
  • Sensorineural
Donor
Antonius Schneider, Ph.D University of Cologne, Institute of Neurophysiology
Primary Reference

Pereverzev A, Mikhna M, Vajna R, Gissel C, Henry M, Weiergräber M, HeschelerJ, Smyth N, Schneider T. Disturbances in glucose-tolerance, insulin-release, and stress-induced hyperglycemia upon disruption of the Ca(v)2.3 (alpha 1E) subunitof voltage-gated Ca(2+) channels. Mol Endocrinol. 2002 Apr;16(4):884-95. (Medline PMID: 11923483)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N4 (C57BL/6J), but background of cre deleter unknown, hence STOCK
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 6 to 8 months
Average litter size
4-6
Recommended wean age
3 Weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050523-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
050523-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.