Strain Name:
FVB/N-Tg(tetO-CNN5,-AcGFP1)2Sban/Mmmh
Stock Number:
050596-MU
Citation ID:
RRID:MMRRC_050596-MU
Other Names:
FVB/N

Strain Information

Tg(tetO-CNN5,-AcGFP1)2Sban
Name: transgene insertion 2, Sushanta Banerjee
Type: Allele
Species: Multi-species
Chromosome: unknown
CNN5
Name: cellular communication network factor 5
Type: Gene
Species: Homo sapiens (human)
Chromosome: 20
AcGFP1
Name: basic (constitutively fluorescent) green fluorescent protein, derived from Aequorea coerulescens
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
tetO
Name: tetracycline-responsive promoter element
Type: Regulatory Region
Species: Multi-species
Chromosome:
Genetic Alterations
This mouse expresses the human CCN5 gene and a green fluorescence gene sequence (Ac-GFP1) under the control of Tet-operator (tetracycline controlled promoter).
Genotype Determination
ES Cell Line
Not specified
Phenotype
None/Normal/Wild-type
Strain Development
The coding sequence of human CCN5 (765 bp) was cloned to the multiple cloning sites positioned downstream of the Tet promoter in a pTRE-Tight BI-AcGFP-1 vector (Takara Bio, Cat#631066). This vector contains a modified TRE and minimal CMV promoter that provide better control of gene expression by eliminating leaky transgene expression in the absence of inducer. Initially, PRK5-hCCN5 vector was obtained from Dr Pennica (Genentech Inc., San Francisco, CA, USA). CCN5 cDNA fragments were amplified by PCR using KpnI and NheI restriction sequences tagged forward and reverse primers, respectively. The PCR generated fragments were cloned into the KpnI and NheI restriction sites of the multiple cloning sites of the vectors. The recombinant clones were verified by restriction digestion analysis, PCR with human CCN5 primers and sequencing. After the preparation of the construct, the founder line was generated by injecting the linearized construct into fertilized oocytes harvested from super-ovulated FVB/N mice. Eight founders were identified. Founder #2 (female) was selected for further breeding and expansion. This female was mated to FVB/N male and progenies were screened for the presence of the transgene. The colony was then backcrossed to FVB/N mice for 12 generations. Mice were then sibling mated after the backcrossing was completed, for at least 150 generations to date.
Suggested Control Mice
Wild-type FVB/N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Immunology and Inflammation
  • Models for Human Disease
  • Obesity
  • Research Tools
Donor
Sushanta Banerjee, Ph.D., VA Medical Center.
Primary Reference

Sarkar S, Ghosh A, Banerjee S, Maity G, Das A, Larson MA, Gupta V, Haque I,Tawfik O, Banerjee SK. CCN5/WISP-2 restores ER-∝ in normal and neoplastic breast cells and sensitizes triple negative breast cancer cells to tamoxifen.Oncogenesis. 2017 May 22;6(5):e340. doi: 10.1038/oncsis.2017.43. (Medline PMID: 28530705)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Sib-mating
Generation
N12 (FVB/N), F150
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Variable Variable
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050596-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
050596-MU-RESUS Litter recovered from cryo-archive $2,697.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.