Strain Name:
B6NJ.Cg-Msratm1Jmo/Mmjax
Stock Number:
050602-JAX
Citation ID:
RRID:MMRRC_050602-JAX
Other Names:
C57BL/6NJtm/MsrAKO

Gene Information

Msratm1Jmo
Name: methionine sulfoxide reductase A; targeted mutation 1, Jackob Moskovitz
Synonyms: MsrA-, MsrA1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Msra
Name: methionine sulfoxide reductase A
Synonyms: 2310045J23Rik, MSR-A, 6530413P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
NCBI: 110265
HGNC: HGNC:7377
Homologene: 5812
Genetic Alterations
The coding sequence in exon 2 (harboring the active site of the MsrA protein) was abolished.
ES Cell Line
RW-4
Phenotype
None/Normal/Wild-type. This mouse doesn’t express methionine sulfoxide reductase A (MSRA). MSRA is an antioxidant enzyme that scavenges intracellular reactive oxygen species (ROS) toxic to the animal. To keep ROS level down, MSRA needs to be expressed to deal with stress.
Mammalian Phenotype Terms
Allelic Composition: Msratm1Jmo/Msratm1Jmo (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

Allelic Composition: Msratm1Jmo/Msratm1Jmo (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

MeSH Terms
  • Animals
  • Antioxidants/metabolism
  • Cell Line
  • Gene Targeting
  • Life Expectancy
  • Methionine/chemistry
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Oxidative Stress
  • Oxidoreductases/chemistry
  • Oxidoreductases/genetics
  • Oxidoreductases/metabolism
  • Oxidoreductases Acting on Sulfur Group Donors
  • Longevity/genetics
  • Methionine Sulfoxide Reductases
  • Mice, Knockout
  • Oxidative Stress/genetics
Strain Development
Exon 2 of gene Msra was targeted in the ES cell line E14.5 RW4, which is derived from mouse 129X1/SvJ blastocysts. Alteration of exon 2 was confirmed via Southern blot in the targeted clones. Homozygous material was used for injection of C57BL/6J blastocysts followed by transfer into pseudopregnant females. Chimeras were mated with C57BL/6J mice for germline transmission (F1 heterozygous mice), which were crossed to produce F2 animals (maintained in homo/hemizygous state). Subsequent backcrossing to C57BL/6NJ, N10.
Suggested Control Mice
C57BL/6NJ
Donor
Earl R. Stadtman, Ph.D., NIH - National Heart, Lung, and Blood Institute.
Rodney L. Levine, M.D., National Heart, Lung, and Blood Institute.
Primary Reference

Salmon, A.B., et al (2000). Lackof methionine sulfoxide reductase A in mice increases sensitivity to oxidativestress but does not diminish life span. FASEB J 23,3061-3068.Salmon, A. B., Perez, V. I., Bokov, A., Jernigan, A., Kim, G., Zhao, H.,Levine R. L., and Richardson, A. (2009).

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Pink
MMRRC Breeding System
Backcross
Generation
N10+ (C57BL/6NJ)
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Variable Variable
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
050602-JAX-HOM-F
050602-JAX-HOM-M
Homozygous Female
Homozygous Male
$218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.