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Strain Detail Sheet

Strain Name:
B6J.129P2(Cg)-Mirc22tm1.1Mtm/Mmucd
Stock Number:
050727-UCD
Citation ID:
RRID:MMRRC_050727-UCD
Other Names:
Mirc22 floxed (aka mir-23b/27b/24-1 floxed)

Strain Information

Gene Details

Mirc22tm1.1Mtm
Name: microRNA cluster 22, including Mir23b through Mir24-1; targeted mutation 1.1, Micheal McManus
Synonyms: Mirc22f
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Conditional
Mirc22
Name: microRNA cluster 22, including Mir23b through Mir24-1
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Conditional
Genetic Alterations
Floxed allele of the miRNA cluster containing miR-23b, miR-27b and miR-24-2. E14 (129P2/OlaHsd) ES cells were targeted as described (PMID: 22570807) and used to generate chimeric mice with a conditionally mutant allele of the cluster containing miR-23b, miR-27b, and miR-24-1 (Mirc22tm1.1Mtm,here Mirc22f). These chimeras were crossed to Rosa26-Flp mice (Gt(ROSA)26Sortm1(FLP1)Dym; 009086, the Jackson Laboratory) to delete the selection cassette.
ES Cell Line
E14

Strain Description

Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • Asthma/immunology
  • Base Sequence
  • Cell Differentiation/genetics
  • Cell Differentiation/immunology
  • Cells, Cultured
  • Disease Models, Animal
  • Female
  • Inflammation/immunology
  • Interleukin-4/biosynthesis
  • Interleukin-4/immunology
  • Lymphocyte Activation/immunology
  • Male
  • Mice
  • Mice, Knockout
  • MicroRNAs/genetics
  • Multigene Family/genetics
  • Sequence Analysis, RNA
  • Th2 Cells/cytology
  • Th2 Cells/immunology
Strain Development
E14 (129P2/OlaHsd) ES cells were targeted as described (PMID: 22570807) and used to generate chimeric mice with a conditionally mutant allele of the cluster containing miR-23b, miR-27b, and miR-24-1 (Mirc22tm1.1Mtm, here Mirc22floxed ).
Suggested Control Mice
WT and/or floxed allele without Cre.

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Cell Biology
  • Immunology and Inflammation

Strain Origin

Donor
K Mark Ansel, University of California, San Francisco.
Primary Reference

Pua HH, Steiner DF, Patel S, Gonzalez JR, Ortiz-Carpena JF, Kageyama R, Chiou NT, Gallman A, de Kouchkovsky D, Jeker LT, McManus MT, Erle DJ, Ansel KM.MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network ofRegulators of T Helper 2 Cell-Associated Cytokine Production. Immunity. 2016 Apr 19;44(4):821-32. doi: 10.1016/j.immuni.2016.01.003. Epub 2016 Feb 2. (Medline PMID: 26850657)

Colony and Husbandry Information

Special Considerations

This line was found to unexpectedly test positive for Cre and therefore may require additional breeding in order to remove the Cre before distribution. This additional breeding would affect the timeline of when mice from this line would be distributed.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Microbiome Analysis

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N=14 (C57BL/6J)
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
050727-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.