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The murine rhodopsin gene, flanked by two thymidine kinase genes was disrupted by introducing a pol2-neo cassette into exon 2. The construct was electroporated into R1 ES cells and positive-negative selected targeted cell lines microinjected into C57BL/6 blastocysts. Chimeric males produced agouti offspring (70%). Heterozygotes were crossed to produce litters of all three rhodopsin genotypes – heterozygotes, homozygotes, and wild-type. Subsequently marker-assisted backcrossing to N12 onto the 129Sv background.
Note: Exact 129Sv substrain unclear.
Humphries MM, Rancourt D, Farrar GJ, Kenna P, Hazel M, Bush RA, Sieving PA,Sheils DM, McNally N, Creighton P, Erven A, Boros A, Gulya K, Capecchi MR,Humphries P. Retinopathy induced in mice by targeted disruption of the rhodopsin gene. Nat Genet. 1997 Feb;15(2):216-9. (Medline PMID: 9020854)
Travis GH. Insights from a lost visual pigment. Nat Genet. 1997Feb;15(2):115-7. (Medline PMID: 9020830)
Toda K, Bush RA, Humphries P, Sieving PA. The electroretinogram of therhodopsin knockout mouse. Vis Neurosci. 1999 Mar-Apr;16(2):391-8. (Medline PMID: 10367972)
Humphries MM, Kiang S, McNally N, Donovan MA, Sieving PA, Bush RA, Machida S, Cotter T, Hobson A, Farrar J, Humphries P, Kenna P. Comparative structural andfunctional analysis of photoreceptor neurons of Rho-/- mice reveal increasedsurvival on C57BL/6J in comparison to 129Sv genetic background. Vis Neurosci.2001 May-Jun;18(3):437-43. (Medline PMID: 11497420)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.