Strain Name:
FVB/N-Tg(Myh6-Pln)416Egk/Mmmh
Stock Number:
000061-MU
Citation ID:
RRID:MMRRC_000061-MU

Strain Information

Pln
Name: phospholamban
Synonyms: PLB
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18821
VEGA: 10
HGNC: HGNC:9080
Homologene: 2002
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alphaMHC, alpha cardiac MHC, A830009F23Rik, cardiomyopathy, hypertrophic 1, Myhc-a, Myhca, alpha-MHC, alpha myosin
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Tg(Myh6-Pln)416Egk
Name: transgene insertion 416, Evangelia G Kranias
Synonyms: TgPLNwt, Tg(Pln)416Egk
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Transgene insertion of mouse phospholamban with alpha-myosin heavy chain 6 promoter.
Genotype Determination
Phenotype
Overexpression of phospholamban results in (a) inhibition of sarcoplasmic reticulum Ca2+ transport; (b) decreases in systolic Ca2+ levels and contractile parameters in ventricular myocytes; and (c) depression of basal left ventricular systolic function in vivo.
MeSH Terms
  • Adrenergic beta-Agonists/pharmacology
  • Animals
  • Calcium/metabolism
  • Calcium-Binding Proteins/metabolism
  • Calcium-Transporting ATPases/metabolism
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Isoproterenol/pharmacology
  • Mice
  • Mice, Transgenic
  • Myocardial Contraction
  • Myocardium/metabolism
  • Receptors, Adrenergic, beta/physiology
  • Sarcoplasmic Reticulum/metabolism
Strain Development
Maintained by intra-strain inbreeding of wildtype and hemizygous littermates.
Suggested Control Mice

wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • The mechanisms of the stimulatory effects of beta-agonists in the healthy heart;
  • The involvement of the beta-adrenergic system in heart disease, where it has been implicated to play a major role.
Donor
Evangelia Kranias, Ph.D., University of Cincinnati, College of Medicine.
Kent Lloyd, D.V.M., University of California, Davis.
Primary Reference

Kadambi VJ, Ponniah S, Harrer JM, Hoit BD, Dorn GW 2nd, Walsh RA, Kranias EG. Cardiac-specific overexpression of phospholamban alters calcium kinetics and resultant cardiomyocyte mechanics in transgenic mice. J Clin Invest. 1996 Jan 15; 97(2):533-9. (Medline PMID: 8567978)

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
White
Eye
Red
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Hemizygous Female x Wildtype Male
  • Wildtype Female x Hemizygous Male
Generation
Unknown
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Bred to Homozygosity
Yes
Average litter size
7
Recommended wean age
3 weeks
Average Pups Weaned
7

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000061-MU-RESUS Litter recovered from cryo-archive $2,028.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.