Strain Name:
NOD.B10Sn-Idd9.3C57BL/10SnJ Tnfrs9em3Ygch/Mmjax
Stock Number:
065427-JAX
Citation ID:
RRID:MMRRC_065427-JAX
Other Names:
NOD.Idd9.3.Tnfrsf9-/-

Strain Information

Tnfrsf9em3Ygch
Name: tumor necrosis factor receptor superfamily, member 9; endonuclease-mediated mutation 3, Yi-Guang Chen
Synonyms: Tnfrsf9-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Endonuclease-mediated
Tnfrsf9
Name: tumor necrosis factor receptor superfamily, member 9
Synonyms: 4-1BB, CDw137, Ly63, Cd137, ILA, A930040I11Rik
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Endonuclease-mediated
NCBI: 21942
Homologene: 1199
Idd9.3C57BL/10SnJ
Name: insulin dependent diabetes susceptibility 9.3; C57BL/10SnJ
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Non-mutant
Idd9.3
Name: insulin dependent diabetes susceptibility 9.3
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Non-mutant
Genetic Alterations
2-bp deletion in the second coding exon of the Tnfrsf9 gene.
MeSH Terms
  • Animals
  • Diabetes Mellitus, Type 1/genetics
  • Diabetes Mellitus, Type 1/immunology
  • Gene Knockout Techniques/methods
  • Mice
  • Mice, Inbred NOD
  • T-Lymphocytes, Regulatory/immunology
  • Tumor Necrosis Factor Receptor Superfamily, Member 9/genetics
  • Tumor Necrosis Factor Receptor Superfamily, Member 9/immunology
  • Zinc Fingers/genetics
  • Zinc Fingers/immunology
Strain Development
Tnfrsf9 was targeted using zinc-finger nuclease (ZFN). ZFN pairs were designed to specifically target the second coding exon of the mouse Tnfrsf9 gene (target sequence CCCTCCAAGTACCTTctccaGCATAGGTGGACAGCCGAA; ZFNs bind to each sequence shown in upper case on opposite strands). mRNAs encoding ZFN pairs were prepared in injection buffer (1mM Tris-Cl, 0.1mM EDTA, pH 7.4) at a concentration between 5-10 ng/µl and injected into the pronucleus of fertilized NOD.B10Sn-Idd9.3C57BL/10SnJ/1106MrkTacJ (JAX Strain #012311) one-cell embryos. Injected embryos were transferred to pseudopregnant CD-1 females. At weaning, DNA was extracted from tail tissues and screened for ZFN-induced mutation. A founder with a two base-pair insertion at the target site, resulting in a premature stop codon, was backcrossed to NOD.B10Sn-Idd9.3C57BL/10SnJ/1106MrkTacJ for 4 generations followed by intercrossing to fix the mutation to homozygosity.
Suggested Control Mice
NOD.Idd9.3
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Diabetes
  • Immunology and Inflammation
Donor
Yi-guang Chen, Ph.D., Medical College of Wisconsin.
Primary Reference

Forsberg MH, Foda B, Serreze DV, Chen YG. Combined congenic mapping andnuclease-based gene targeting for studying allele-specific effects of Tnfrsf9within the Idd9.3 autoimmune diabetes locus. Sci Rep. 2019 Mar 13;9(1):4316. doi:10.1038/s41598-019-40898-8. PubMed PMID: 30867509; PubMed Central (Medline PMCID: PMC6416332)

Chen YG, Forsberg MH, Khaja S, Ciecko AE, Hessner MJ, Geurts AM. Genetargeting in NOD mouse embryos using zinc-finger nucleases. Diabetes. 2014Jan;63(1):68-74. doi: 10.2337/db13-0192. Epub 2013 Aug 23. (Medline PMID: 23974926)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Sib-mating
Generation
N4 (NOD.B10Sn)
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
065427-JAX-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
065427-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.