Strain Name:
FVB.129P2-Kat5tm2Tfaz/Mmmh
Stock Number:
065439-MU
Citation ID:
RRID:MMRRC_065439-MU
Other Names:
KAT5(Tip55)

Strain Information

Kat5tm2Tfaz
Name: K(lysine) acetyltransferase 5; targeted mutation 2, Thomas Fazzio
Synonyms: Tip55-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Targeted Mutation
Kat5
Name: K(lysine) acetyltransferase 5
Synonyms: PLIP, Tip60, Htatip, mHTATIP/CPLA2
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Targeted Mutation
NCBI: 81601
VEGA: 19
HGNC: HGNC:5275
Homologene: 100661
Genetic Alterations
Specific knockout of the Tip55 isoform of Kat5 by targeting a construct to the Kat5 locus that fuses the last four exons and removes the intervening introns, one of which is retained in the Tip55 transcript. The resulting mouse expresses all other Kat5 isoforms normally but not Tip55.
Genotype Determination
ES Cell Line
E14-Tg2A
Phenotype
Heterozygotes have no phenotype. Homozygotes are embryonic lethal, exhibiting defects in the heart and neural tube, and dying between embryonic days 10.5 and 12.5.
Strain Development
Targeting vector based on pBlueScript flanking a LoxP-pGK puromycin-LoxP selection cassette containing a BAC clone that contains exons 11-14 of murine gene Tip60 (NCBI Gene ID: 81601, NM_001199247). Electroporation of linearized target vector into E14-Tg2A ES cells (origin: 129P2). Puromycin selection, clonal expansion, and screening via PCR for correct targeting into the Tip60 exon 11 locus. This allele specifically lacks the Tip55 isoform. Three ES clones injected into blastocysts; implantation into FVB female to produce chimeras. Tail-snip PCR to confirm the genotype of chimeras. Mating of chimera offspring with FVB/N for germline transmission.
Suggested Control Mice
FVB/N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Developmental Biology
Donor
Thomas Fazzio, Ph.D., University of Massachusetts Medical School.
Primary Reference

Acharya D, Nera B, Milstone ZJ, Bourke L, Yoon Y, Rivera-Pérez JA, Trivedi CM,Fazzio TG. TIP55, a splice isoform of the KAT5 acetyltransferase, is essentialfor developmental gene regulation and organogenesis. Sci Rep. 2018 Oct8;8(1):14908. doi: 10.1038/s41598-018-33213-4. (Medline PMID: 30297694)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Backcross
Generation
N9 (FVB/N)
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months Greater than 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
065439-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
065439-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.