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None/Normal/Wild-type
Conditional phenotype: In the absence of tetracycline, the suppressor binds to tet operator binding sites thereby suppressing transcription from any promoter containing these sites. In the presence of tetracycline, the transgene will not bind the target tet operator sites.
To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.
The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.
Richardson-Jones JW, Craige CP, Guiard BP, Stephen A, Metzger KL, Kung HF, Gardier AM, Dranovsky A, David DJ, Beck SG, Hen R, Leonardo ED. 5-HT1Aautoreceptor levels determine vulnerability to stress and response to antidepressants. Neuron. 2010 Jan 14;65(1):40-52. doi:10.1016/j.neuron.2009.12.003. PubMed PMID: 20152112; PubMed Central (Medline PMCID: PMC2941196)
Audero E, Coppi E, Mlinar B, Rossetti T, Caprioli A, Banchaabouchi MA, Corradetti R, Gross C. Sporadic autonomic dysregulation and death associated with excessive serotonin autoinhibition. Science. 2008 Jul 4;321(5885):130-3. doi:10.1126/science.1157871. (Medline PMID: 18599790)
Scott MM, Wylie CJ, Lerch JK, Murphy R, Lobur K, Herlitze S, Jiang W, ConlonRA, Strowbridge BW, Deneris ES. A genetic approach to access serotonin neurons for in vivo and in vitro studies. Proc Natl Acad Sci U S A. 2005 Nov8;102(45):16472-7. Epub 2005 Oct 26. (Medline PMID: 16251278)
Gross C, Zhuang X, Stark K, Ramboz S, Oosting R, Kirby L, Santarelli L, Beck S, Hen R. Serotonin1A receptor acts during development to establish normal anxiety-like behaviour in the adult. Nature. 2002 Mar 28;416(6879):396-400. (Medline PMID: 11919622)
Dietrich P, Dragatsis I, Xuan S, Zeitlin S, Efstratiadis A. Conditional mutagenesis in mice with heat shock promoter-driven cre transgenes. Mamm Genome. 2000 Mar;11(3):196-205. (Medline PMID: 10723724)
Deuschle U, Meyer WK, Thiesen HJ. Tetracycline-reversible silencing of eukaryotic promoters. Mol Cell Biol. 1995 Apr;15(4):1907-14. (Medline PMID: 7891684)
Tg(Fev-tTs)1Leod was produced by cloning the coding sequence of tTS protein followed by an SV40 polyadenylation signal (PMID:7891684) into the T3 polylinker region of the NarI/BgzA modification 5 plasmid, placing the coding sequence downstream of a b-globin promoter (PMID:16251278). The b-globin promoter and tTS coding sequence were then released with an RsrII digest and the resulting fragment was cloned into the EPet-1Mini-BACMod#1 containing a 40-kb genomic DNA fragment (540z) that lies upstream of the Fev coding sequence (PMID:16251278). The resulting BAC EPet-1-tTs was linearized and subjected to pronuclear injection into B6CBAF2 hybrid zygotes. Founders were identified by PCR. Animals harboring the Tg(Fev-tTs)1Leod allele as well as the Htr1atm3.1Rhn allele (Cre deleter background unknown) were generated by breeding Tg(Fev-tTs)1Leod mice onto the Htr1atm3.1Rhn background. Tg(Fev-tTs)1Leod/Htr1atm3.1Rhn males were then bred to Htr1atm3.1Rhn females. As a result, the Tg(Fev-tTs)1Leod transgene was transmitted through the male germline, ensuring that all pups were raised by mothers of the same genotype, regardless of doxycycline status. The line was backcrossed to the 129S6/SvEvTac more than four times. Upon arrival at the MMRRC, 129S1 oocytes were used, moving the genetic background designation to stock.
Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.
When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
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