Strain Name:
C57BL/6J-In(X4930567H17Rik)1Jbmu/Mmnc
Stock Number:
065555-UNC
Citation ID:
RRID:MMRRC_065555-UNC
Other Names:
In(X4930567H17Rik)1Jbmu

Strain Information

In(X4930567H17Rik)1Jbmu
Name: inversion, Chr X, RIKEN cDNA 4930567H17 gene; Jacob Mueller 1er
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
4930567H17Rik
Name: RIKEN cDNA 4930567H17 gene
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 619303
Homologene: 134521
Genetic Alterations
Inversion of one of two palindrome arms, each of which harbors a copy of 4930567H17Rik.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Unknown
    MeSH Terms
    • Animals
    • Chromosome Deletion
    • Chromosome Inversion
    • Gene Expression Regulation
    • Inverted Repeat Sequences
    • Male
    • Meiosis
    • Mice
    • X Chromosome/genetics
    • X Chromosome/metabolism
    Strain Development
    To generate mice carrying palindrome arm inversions the donor used CRISPR with dual single guide RNAs (sgRNAs). sgRNAs were designed to unique sequences flanking the targeted palindrome arm, as close to the edge of the arm as possible. After active sgRNAs were identified for both sides of each targeted palindrome arm, two pX458M plasmids encoding the sgRNAs and Cas9 were injected into the pronucleus of B6JSJLF1 mouse zygotes. A single-stranded oligonucleotide with sequence homology flanking the junction boundaries to promote the event was added. The zygotes were generated from mating C57BL/6J females to B6JSJLF1 males so that all targeted X chromosomes were C57BL/6J. Blastocysts from the pronuclear injection were implanted into pseudopregnant females. Genomic DNA from resulting pups was screened by PCR and Sanger sequencing of the deletion junctions. Male and female mice were able to transmit the inversion through the germline.
    Suggested Control Mice
    wild-type littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    Donor
    Jacob Mueller, Ph.D., University of Michigan Medical School.
    Primary Reference

    Kruger AN, Ellison Q, Brogley MA, Gerlinger ER, Mueller JL. Male mice withlarge inversions or deletions of X-chromosome palindrome arms are fertile andexpress their associated genes during post-meiosis. Sci Rep. 2018 Jun12;8(1):8985. doi: 10.1038/s41598-018-27360-x. (Medline PMID: 29895860)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross
    Generation
    N10 (C57BL/6J)
    Overall Breeding Performance
    Good
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes X-linked
    Homozygotes are fertile: Yes X-linked
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    065555-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.