Strain Name:
B6J.B6N-Kif17tm1a(EUCOMM)Wtsi/BjcMmucd
Stock Number:
065556-UCD
Citation ID:
RRID:MMRRC_065556-UCD
Other Names:
Osm-3/Kif17tm1a(Bjc)

Strain Information

Kif17tm1a(EUCOMM)Wtsi
Name: kinesin family member 17; targeted mutation 1a, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Kif17
Name: kinesin family member 17
Synonyms: Kif17b, 5930435E01Rik, N-4 kinesin
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knockout
NCBI: 16559
Homologene: 5883
Genetic Alterations
The L1L2_Bact_P targeting cassette contains a floxed exon 4 of mouse Kif17 along with an upstream lacZ reporter. Targeted cells purchased from EUCOMM were used to make germline mice.
Genotype Determination
ES Cell Line
JM8.F6
Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • Base Sequence
  • CRISPR-Cas Systems/genetics
  • Cilia/drug effects
  • Cilia/metabolism
  • Gene Editing
  • Hedgehog Proteins/metabolism
  • Kinesin/metabolism
  • Mice, Inbred C57BL
  • Models, Biological
  • Morphogenesis/drug effects
  • Morpholinos/pharmacology
  • Mutation/genetics
  • Neurogenesis/drug effects
  • Retinal Photoreceptor Cell Outer Segment/metabolism
  • Signal Transduction/drug effects
  • Temperature
  • Transcription Activator-Like Effector Nucleases
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
Strain Development
The Kif17 mutant mice were generated using targeted C57BL/6N stem cells (JM8.F6) obtained through EUCOMM. The L1L2_Bact_P targeting cassette contained a floxed exon 4 of mouse Kif17 along with an upstream lacZ reporter. Targeted cells purchased from EUCOMM were used to make germline mice in the Medical College of Wisconsin Transgenic Core. Germ-line mice were mated to C57BL/6J mice for 5 generations.
Suggested Control Mice
Littermate
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Models for Human Disease
Donor
Joseph C. Besharse, Ph.D., Medical College of Wisconsin.
Primary Reference

Lewis TR, Kundinger SR, Pavlovich AL, Bostrom JR, Link BA, Besharse JC.Cos2/Kif7 and Osm-3/Kif17 regulate onset of outer segment development inzebrafish photoreceptors through distinct mechanisms. Dev Biol. 2017 May15;425(2):176-190. doi: 10.1016/j.ydbio.2017.03.019. Epub 2017 Mar 22. PubMedPMID: 28341548; PubMed Central (Medline PMCID: PMC5558849)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross and random intra-strain mating
Generation
N5 (C57BL6J)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
4 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
065556-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
065556-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.