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Availability & Fees Order this Strain
Sv2a and Sv2b KO lines were backcrossed onto C57BL/6 for 12 generations and these two lines were used to generate Sv2a-/+/Sv2b-/- breeders. The Sv2a-/+/Sv2b-/- breeders were used and bred together to generate the (non-viable) Sv2a/b double KO mice.
Sv2a allele: A portion of the Sv2a gene was isolated from a mouse 129Sv genomic library (Stratagene) by screening with a probe encoding bases 2400 to 1212 of the rat SV2A cDNA (3). A fragment of '12.5 kb was isolated that contained the exon encoding the translation start site through most of the first transmembrane domain of the Sv2a cDNA. Mouse Sv2a sequence was approximately 95% identical to rat in this region. The location of the exon in the genomic fragment was identified by restriction enzyme mapping and Southern analysis. A targeting construct was generated in pBlueScript in which the exon and surrounding DNA were replaced with a gene encoding neomycin resistance. DNA encoding thymidine kinase was placed at the end of the short arm of the targeting construct to allow for negative selection of homologous recombination by using the antiviral agent Gancyclovir (Syntex, Palo Alto, CA). Embryonic stem cells were transfected with linearized targeting construct by electroporation. Cells were placed under selection in G418 (GIBCOyBRL) and Gancyclovir. Resistant colonies were screened for homologous recombination by Southern analysis. Four cell lines carrying the Sv2a disruption were injected into C57BL/6 blastocysts and implanted into pseudopregnant females. One of ten chimeric males produced offspring heterozygous for the Sv2a gene disruption. These offspring were bred with each other to produce animals homozygous for the mutation. Wild-type offspring of heterozygotes were used to establish a colony of genetically matched control animals
Crowder KM, Gunther JM, Jones TA, Hale BD, Zhang HZ, Peterson MR, Scheller RH,Chavkin C, Bajjalieh SM. Abnormal neurotransmission in mice lacking synapticvesicle protein 2A (SV2A). Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15268-73.(Medline PMID: 10611374)
Morgans CW, Kensel-Hammes P, Hurley JB, Burton K, Idzerda R, McKnight GS,Bajjalieh SM. Loss of the Synaptic Vesicle Protein SV2B results in reducedneurotransmission and altered synaptic vesicle protein expression in the retina. PLoS One. 2009;4(4):e5230. doi: 10.1371/journal.pone.0005230. Epub 2009 Apr 17.(Medline PMID: 19381277)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.