Strain Detail Sheet

Strain Name:
B6J;B6N-Syt1em1Thore/Mmmh
Stock Number:
065805-MU
Citation ID:
RRID:MMRRC_065805-MU
Other Names:
Syt1 flox

Strain Information

Gene Details

Syt1em1Thore
Name: synaptotagmin I; endonuclease-mediated mutation 1, Wallace Thoreson
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: CRISPR
Syt1
Name: synaptotagmin I
Synonyms: G630098F17Rik
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: CRISPR
NCBI: 20979
Homologene: 4122
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Genetic Alterations
Conditional, Cre-dependent Syt1 knockout mouse line with LoxP sites flanking exon 6 of Syt1, created using Easi-CRISPR.

Strain Description

Phenotype
Conditional, Cre-dependent synaptotagmin1 (Syt1) knockout mouse line with loxP sites flanking exon 6 of Syt1. Cre-dependent elimination of Syt1 leads to loss of fast synaptic transmission.

Conditional phenotype: Loss of the exocytotic Ca2+ sensor synaptotagmin 1. Cre-dependent elimination of Syt1 leads to loss of fast synaptic transmission at most synapses. Global elimination of Syt1 is lethal within 48 hrs of birth.

MeSH Terms
  • Animals
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • DNA, Single-Stranded/genetics
  • DNA, Single-Stranded/metabolism
  • Endonucleases/genetics
  • Endonucleases/metabolism
  • Founder Effect
  • Gene Editing/methods
  • Genes, Reporter
  • Genetic Loci
  • Integrases/genetics
  • Integrases/metabolism
  • Mice
  • Mice, Transgenic/genetics
  • Mice, Transgenic/growth & development
  • Microinjections
  • Mutagenesis, Insertional/methods
  • RNA, Guide/genetics
  • RNA, Guide/metabolism
  • Recombinational DNA Repair
  • Ribonucleoproteins/genetics
  • Ribonucleoproteins/metabolism
  • Zygote/growth & development
  • Zygote/metabolism
Strain Development
Easi-CRISPR strategy: The donor generated a 635-base ssDNA donor containing a floxed version of exon 6, flanked by two 75-base left and right homology arms. The two parts of the CRISPR guide RNA (crRNA + tracrRNA) and Cas9 protein were combined to generate a ctRNP complex. The long ssDNA donor derived from a floxed exon cassette was mixed with ctRNP(s) to obtain the final Easi-CRISPR reagent cocktail for zygote injection. This was followed by injection of a floxed ssDNA donor with right and left ctRNPs into zygotes resulting in the replacement of the target exon with the floxed exon. Subsequently, microinjection of the Easi-CRISPR reagent cocktail, genotyping, and sequencing were used to identify founders with correctly modified genomes.
Suggested Control Mice
Cre-negative Syt1fl/fl on C57BL/6J background.

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Neurobiology
  • Sensorineural

Strain Origin

Donor
Wallace B. Thoreson, Ph.D., University of Nebraska Medical Center.
Primary Reference

Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T, Redder R,Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL, Ohtsuka M,Gurumurthy CB. Easi-CRISPR: a robust method for one-step generation of micecarrying conditional and insertion alleles using long ssDNA donors and CRISPRribonucleoproteins. Genome Biol. 2017 May 17;18(1):92. doi:10.1186/s13059-017-1220-4. (Medline PMID: 28511701)

Grassmeyer JJ, Cahill AL, Hays CL, Barta C, Quadros RM, Gurumurthy CB,Thoreson WB. Ca(2+) sensor synaptotagmin-1 mediates exocytosis in mammalianphotoreceptors. Elife. 2019 Jun 7;8. pii: e45946. doi: 10.7554/eLife.45946.(Medline PMID: 31172949)

Colony and Husbandry Information

Special Considerations

If crossed with a global Cre deleter, pups survive less than 48 hrs after birth. Targeting cells and tissues with a more specific Cre recombinase is recommended.

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
4 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.