Strain Name:
C57BL/6-Crb1+ Kmotm1a(KOMP)Wtsi/NagarMmjax
Stock Number:
065816-JAX
Citation ID:
RRID:MMRRC_065816-JAX
Other Names:
Kmotm1a(KOMP)Wtsi Crb1WT

Strain Information

Kmotm1a(KOMP)Wtsi
Name: kynurenine 3-monooxygenase; targeted mutation 1a, Wellcome Trust Sanger Institute
Synonyms: Kmonull
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout-first
Kmo
Name: kynurenine 3-monooxygenase
Synonyms: kynurenine 3-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout-first
NCBI: 98256
HGNC: HGNC:6381
Homologene: 2729
Crb1+
Name: crumbs family member 1, photoreceptor morphogenesis associated; wild-type
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout-first
Crb1
Name: crumbs family member 1, photoreceptor morphogenesis associated
Synonyms: A930008G09Rik, 7530426H14Rik
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout-first
NCBI: 170788
HGNC: HGNC:2343
Homologene: 8092
Genetic Alterations
Conditional-ready null/knockout of the Kmo gene: The L1L2_Bact_P cassette was inserted at position 175,643,107 of Chromosome 1 upstream of the critical exons (Build GRCm38); KO mice on a C57BL/6N background were generated by The KOMP Repository. The animals were found to harbor the rd8 mutation in the Crb1 gene (Crb1rd8 - MGI:2676366, PMID:22447858). To make the Kmo KO animals Crb1 wild-type, they were backcrossed with C57BL/6J (N4). Subsequent sib-mating (F5+). Animals are not CRE'ed or FLP'ed.
Genotype Determination
ES Cell Line
Not applicable
Phenotype
Altered Trp metabolism; Increased Kyn, AA, and KYNA, decreased downstream KMO metabolites. Somewhat elevated blood glucose.

Conditional phenotype: The line retains the L1L1_Bact_P cassette. The animals are not CRE'd or FLP'd

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
Kmo KO mice on a C57BL/6N background were generated by and obtained from The KOMP Repository. Animals were crossed with C57BL/6J animals wild-type for Crb1 to remove the rd8 mutation of these Kmo KO, thanks to homologous recombination, over several generations of cross-breeding. Subsequent sib-mating to F5+ and intra "strain" mating of several initial pairs of Kmotm1a(KOMP)Wtsi followed by backcrossing to C57BL/6J (N4). Animals are on a mixed B6J;B6N genetic background
Suggested Control Mice
The donor used C57BL6J for ophthalmic research. One may consider using heterozygous breeding pairs and using Kmo+/+ littermates as control for Kmo-/- experimental animals. Note: There may still be some variation due to the B6J;B6N mixed background, especially once the homozygous animals are bred with C57BL6J to heterozygosity.
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
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Donor
Ram Nagaraj, Ph.D., University of Colorado, School of Medicine.

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
N4 (C57BL/6J), F5+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Undetermined Undetermined
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
065816-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
065816-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.