Strain Detail Sheet

Strain Name:
C57BL/6J-Tg(Vav1-Hottip)1Xumsa/Mmjax
Stock Number:
065946-JAX
Citation ID:
RRID:MMRRC_065946-JAX
Other Names:
Vav1-Hottip-Tg,C57BL/6J-Cdh23Tg(Vav1-Hottip)1Xumsa/Mmjax

Strain Information

Gene Details

Tg(Vav1-Hottip)1Xumsa
Name: transgene insertion 1, Mingjiang Xu
Synonyms: Hottip-Tg
Type: Allele
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (random, gene disruption)
Cdh23
Name: cadherin related 23 (otocadherin)
Synonyms: nmf112, 4930542A03Rik, USH1D, sals, ahl, bob, mdfw, nmf252, nmf181
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 22295
Homologene: 11142
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Vav1
Name: vav 1 oncogene
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 22324
Homologene: 3961
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Hottip
Name: Hoxa distal transcript antisense RNA
Synonyms: Gm15053, ENSMUSG00000055408
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 791364
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Random insertion of a transgene where the mouse Vav1 promoter drives the expression of mouse Hottip. The integration position of the transgene has found to be in the intron between exon 3 and exon 4 of the Cdh23 gene. Mice develop anemia, splenomegaly, increased numbers of immature myeloid and die prematurely. These mice may be useful for the study of acute myeloid leukemia.

Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype
Some of the mice may develop hematopoietic malignancy after about 1 year.
MeSH Terms
  • Animals
  • Cell Proliferation/genetics
  • Cell Self Renewal/genetics
  • Chromatin/metabolism
  • Gene Expression Regulation, Leukemic/genetics
  • Gene Knockdown Techniques/methods
  • Hematopoietic Stem Cells/metabolism
  • Homeodomain Proteins/genetics
  • Leukemia, Myeloid, Acute/genetics
  • Mice
  • RNA, Long Noncoding/genetics
Strain Development
The Hottip-Tg transgenic construct contains the complete coding region sequence of the mouse Hottip locus under the control of the vav 1 oncogene promoter/control regions (Vav1; from the HS321/45-vav vector). The linearized construct was microinjected into the pronuclei of fertilized C57BL/6J. The transgene integrated into chromosome 10 (Chr10:60117191), within the intron between exon 3 and exon 4 of Cdh23 (cadherin 23 (otocadherin) gene. Founder line 1 was established and maintained on the C57BL/6J background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (JAX Stock No. 000664).
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Models for Human Disease

Strain Origin

Donor
Mingjiang Xu, M.D., Mays Cancer Center at University of Texas, Health San Antonio.
Primary Reference

Luo H, Zhu G, Xu J, Lai Q, Yan B, Guo Y, Fung TK, Zeisig BB, Cui Y, Zha J, Cogle C, Wang F, Xu B, Yang FC, Li W, So CWE, Qiu Y, Xu M, Huang S. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice. Cancer Cell. 2019 Dec 9;36(6):645-659.e8. doi: 10.1016/j.ccell.2019.10.011. Epub 2019 Nov 27. (Medline PMID: 31786140)

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N6+ (C57BL/6J, F10
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.