Strain Name:
B6N.129S7-Reretm1Dsco/Mmucd
Stock Number:
066432-UCD
Citation ID:
RRID:MMRRC_066432-UCD
Other Names:
Rere conditional knockout

Gene Information

Reretm1Dsco
Name: arginine glutamic acid dipeptide (RE) repeats; targeted mutation 1, Daryl Scott
Synonyms: Rereflox
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Conditional
Rere
Name: arginine glutamic acid dipeptide (RE) repeats
Synonyms: 1110033A15Rik, Atr2, eyem03Jus, atrophin-2, eyes3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Conditional
NCBI: 68703
HGNC: HGNC:9965
Homologene: 8101
Genetic Alterations
The second coding exon of Rere has been flanked by loxP sites. Recombination between these sites leads to the deletion of that allele and the generation of a null allele.
ES Cell Line
AB2.2 (129S7/SvEvBrd-Hprtb-m2)
Phenotype
This strain contains a floxed allele of Rere. A knockout in endothelial cells leads to the development of ventricular septal defects. We have shown that Rere is critical for normal growth, development of the brain, palate, eye, heart, and kidney in mice. Similar features are seen in humans who are deficient for RERE.

Conditional phenotype: Yes. This strain carries a Rere flox allele that can be used to generate tissue-specific knock-outs.

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MeSH Terms
  • Alleles
  • Animals
  • Carrier Proteins/metabolism
  • Cell Proliferation
  • Embryo, Mammalian/metabolism
  • Endocardial Cushions/embryology
  • Endocardial Cushions/metabolism
  • Endocardial Cushions/pathology
  • Endocardium/embryology
  • Endocardium/metabolism
  • Endocardium/pathology
  • Epithelial-Mesenchymal Transition/genetics
  • GATA4 Transcription Factor/genetics
  • GATA4 Transcription Factor/metabolism
  • Gene Expression Regulation, Developmental
  • Heart Septal Defects, Ventricular/embryology
  • Heart Septal Defects, Ventricular/genetics
  • Mesoderm/pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NIH 3T3 Cells
  • Nerve Tissue Proteins/deficiency
  • Nerve Tissue Proteins/genetics
  • Repressor Proteins/deficiency
  • Repressor Proteins/genetics
Strain Development
The Rere flox allele was generated using recombineering. Briefly, a 9.6-kb fragment containing the second coding exon of Rere was retrieved into a pBluescript-derived plasmid containing 500-bp homology arms from BAC clone bMQ386d15 selected from the M37- 129AB22 BAC library.

A neomycin resistance cassette flanked by two loxP sites was then inserted 5′ to the second coding exon of Rere. A neomycin resistance cassette flanked by an Frt site, and an Frt and loxP site marked with a BglII restriction site was then inserted 3′ to the second coding exon of Rere to generate the final targeting vector. This targeting vector was linearized and electroporated into AB2.2 ES cells (129S7/SvEvBrd-Hprtb-m2). Positive clones were identified by Southern blotting, injected into C57BL/6N blastocysts, and implanted into ICR outbred pseudopregnant females. Highly chimeric males were mated to C57BL/6N females, and agouti pups were selected and genotyped for transmission of the Rere neo allele. In preparation for experiments on a C57BL/6N background, the B6N;129S7 mice bearing the Rere flox allele were backcrossed for at least another six generations onto the C57BL/6N background.
Suggested Control Mice
C57BL/6N wild type animals or, for conditional studies, Rere flox bearing animals without a Cre-expressing allele.
  • Cardiovascular
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Neurobiology
  • Research Tools
  • Sensorineural
Donor
Daryl Scott, M.D., Baylor College of Medicine.
Primary Reference

Kim BJ, Zaveri HP, Jordan VK, Hernandez-Garcia A, Jacob DJ, Zamora DL, Yu W,Schwartz RJ, Scott DA. RERE deficiency leads to decreased expression of GATA4 andthe development of ventricular septal defects. Dis Model Mech. 2018 Aug 28;11(9).pii: dmm031534. doi: 10.1242/dmm.031534. PubMed PMID: 30061196; PubMed Central(Medline PMCID: PMC6176990)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross then sib-mating
Generation
N6+ (C57BL/6)
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
4 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
066432-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
066432-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $7,533.58
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.