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Strain Detail Sheet

Published: 12/11/2019
Revised: 12/17/2019

Strain Name:
IHDID1/Mmmh
Stock Number:
066498-MU
Citation ID:
RRID:MMRRC_066498-MU
Other Names:
inbred High Drinking in the Dark - replicate 1 (iHDID-1)

Strain Information

Gene Details

Genetic Alterations
This is an inbred strain (IHDID1) developed from the selectively bred line HDID-1 (MMRRC:65277), which was developed to increase the blood ethanol concentration (BEC) achieved after drinking 20% alcohol during a 4-hour period. There is no single allele responsible. Rather, the HDID model is shaped by the influence of many genes.
Genotype Determination
There is no genotyping protocol.
ES Cell Line
Not applicable

Strain Description

Phenotype
Mice were bred to voluntarily drink alcohol to the point of intoxication as measured by blood ethanol concentration (BEC) during a 4-hour period.
MeSH Terms
  • Animals
  • Binge Drinking/genetics
  • Ethanol/blood
  • Female
  • Inbreeding
  • Male
  • Mice
  • Selection, Genetic
  • Alcohol Drinking/genetics
  • Alcoholic Intoxication/genetics
  • Darkness
  • Disease Models, Animal
  • Mice, Inbred Strains/genetics
  • Mice, Inbred Strains/metabolism
  • Mice, Inbred Strains/psychology
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Models for Human Disease

Strain Origin

Submitter
John Crabbe, Ph.D., Oregon Health & Science University.
Primary Reference

Crabbe JC, Metten P, Savarese AM, Ozburn AR, Schlumbohm JP, Spence SE, HackWR. Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice. BrainSci. 2019 Jan 1;9(1). pii: E2. doi: 10.3390/brainsci9010002. PubMed PMID:30609665; PubMed Central (Medline PMCID: PMC6356868)

Crabbe JC, Metten P, Belknap JK, Spence SE, Cameron AJ, Schlumbohm JP, HuangLC, Barkley-Levenson AM, Ford MM, Phillips TJ. Progress in a replicated selectionfor elevated blood ethanol concentrations in HDID mice. Genes Brain Behav. 2014Feb;13(2):236-46. doi: 10.1111/gbb.12105. Epub 2013 Dec 6. PubMed PMID: 24219304;PubMed Central (Medline PMCID: PMC3923418)

Crabbe JC, Metten P, Rhodes JS, Yu CH, Brown LL, Phillips TJ, Finn DA. A line of mice selected for high blood ethanol concentrations shows drinking in the darkto intoxication. Biol Psychiatry. 2009 Apr 15;65(8):662-70. doi:10.1016/j.biopsych.2008.11.002. Epub 2008 Dec 18. (Medline PMID: 19095222)

Strain Development
See MMRRC:65277 for details about selective breeding from the HS/Npt founder population. To generate the inbred line, HDID mice (MMRRC:65277) at selected generation 26 were sib-mated for 22 generations.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
White
Eye
Pink
Other
This inbred line is one of two genetically independent replicate lines bred for high blood ethanol concentrations (BEC) after a procedure known as "drinking in the dark." This procedure offered the mice a 2-hour period in which to drink 20% v/v ethanol in tap water 3 hours into their dark cycle on Day 1. On Day 2, the period of access to ethanol was extended to 4 hours, with readings of consumption at 2 and at 4 hours. Immediately after reading the final consumption amount, the mice were bled (20 µl) from the periorbital sinus. This blood sample was assayed for BEC by gas chromatography, and the mice of each sex selected for breeding the next generation had the top 15-20 BECs.

At the time of donation, this was the first selective breeding project that used a blood-drug value for the selection index rather than a behavior or physical characteristic. Furthermore, this unique genetic animal model drank to higher blood ethanol concentrations than the standard inbred strain, C57BL/6J, that had been the highest preferring animal model. Importantly, this drinking-in-the-dark model has been adopted by many laboratories to mimic binge-like drinking in mice, and it has been adapted for other rodents (rats, voles, and hamsters). It is simple, minimally invasive, and does not require alcohol adulteration, thirst motivation, long periods of ramping up the ethanol concentration, administration by gavage, or other manipulations. The ethanol tube is placed on the cage of an individually-housed mouse and the water is removed. When the drinking period is ended, water is restored. The mouse may choose whether or not to drink the ethanol.

Embryo cryopreservation was required, as the background "strain" is not an inbred strain, but rather a heterogeneous stock created from an 8-way inbred strain cross, and maintained by a strict, within-family, rotational mating scheme among 48 families (to reduce inbreeding).

Breeding

MMRRC Breeding System
Sib-mating
Generation
F22
Overall Breeding Performance
Poor

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
066498-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit 		Aliquot Approximate quantity2 : 20-40 embryos / aliquot
066498-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit 		Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.