Strain Name:
129S1.B6-ApcMin Atp5f1aMom2/+ +/LdasMmmh
Stock Number:
066519-MU
Citation ID:
RRID:MMRRC_066519-MU
Other Names:
129.B6 ApcMin Atp5a1Mom2R/ + +

Strain Information

ApcMin
Name: APC, WNT signaling pathway regulator; multiple intestinal neoplasia
Synonyms: ApcΔ850, Apcdelta850, Min-, Apc580S, Apc-, Min
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: adenomatosis polyposis coli, Min, CC1
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Atp5f1aMom2
Name: ATP synthase F1 subunit alpha; Modifier of Min 2
Synonyms: Mom2R, Atp5a1Mom2-C57BL/6J
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Spontaneous Mutation
Atp5f1a
Name: ATP synthase F1 subunit alpha
Synonyms: Mom2, D18Ertd206e, Atp5a1, Atpm
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Spontaneous Mutation
NCBI: 11946
HGNC: HGNC:823
Homologene: 2985
Genetic Alterations
The ApcMin allele resulted from chemical mutagenesis. The Atp5a1Mom2 allele was the result of a spontaneous mutation. Four ApcMin Atp5a1Mom2R/ + + males from the donor's colony were genotyped using MUGA; the only regions found to be B6 in origin were the regions immediately flanking the ApcMin and Atp5a1Mom2 mutations.
Phenotype
These mice develop only a few polyps in their small and large intestines and are long-lived.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Chromosome Mapping
  • Fetal Death/genetics
  • Genetic Linkage
  • Intestinal Polyposis/enzymology
  • Intestinal Polyposis/genetics
  • Intestinal Polyposis/prevention & control
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mitochondrial Proton-Translocating ATPases/genetics
  • Mutation
  • Phenotype
  • Polyps/genetics
  • Tumor Suppressor Proteins/genetics
Strain Development
C57BL/6J-ApcMin Atp5a1Mom2/+ + was backcrossed to 129S1/SvImJ for more than 20 generations. The colony was then maintained by intercrossing (mostly, but not exclusively) wild-type females with ApcMin Atp5a1Mom2/+ + males.
Suggested Control Mice
129.B6 + +/+ + mice from this colony
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Developmental Biology
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Research Tools
Donor
Linda Siracusa, Ph.D., Seton Hall University, Hackensack Meridian School of Medicine.
Primary Reference

Baran AA, Silverman KA, Zeskand J, Koratkar R, Palmer A, McCullen K, Curran WJJr, Edmonston TB, Siracusa LD, Buchberg AM. The modifier of Min 2 (Mom2) locus:embryonic lethality of a mutation in the Atp5a1 gene suggests a novel mechanismof polyp suppression. Genome Res. 2007 May;17(5):566-76. Epub 2007 Mar 26. (Medline PMID: 17387143)

Colony and Husbandry Information

Animals of this strain like to hide but breed well on Aspen shred bedding. Mating cages were given shepherd shacks and nestlets.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Generation
N20+ (129S1/SvImJ)
F7-11
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
066519-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
066519-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.