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Strain Detail Sheet

Published: 02/19/2020
Revised: 04/21/2020

Strain Name:
FVB/NJ-Tg(Myh6-UCHL1)248B1Cuit/Mmnc
Stock Number:
066727-UNC
Citation ID:
RRID:MMRRC_066727-UNC
Other Names:
CR-Uchl1-tg

Strain Information

Gene Details

Tg(Myh6-UCHL1)248B1Cuit
Name: transgene insertion 248B1, Taixing Cui
Type: Allele
Species: Multi-species
Chromosome: unknown
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 15
UCHL1
Name: ubiquitin C-terminal hydrolase L1
Type: Gene
Species: Homo sapiens (human)
Chromosome: 4
Genetic Alterations
Transgenic mice with cardiomyocyte-specific overexpression of human UCHL1 were generated in an FVB/NJ background using a transgene cassette containing a rat 5.5-kb alpha myosin heavy chain promoter.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.

    • Strain Description

    Phenotype
    UCHL1 is a deubiquitinating enzyme (DUB) but its functional importance and substrate proteins are still poorly understood. CR-UCHL1-tg mice are easy breeders with standard genotyping; they constitute a useful tool in cardiac disease. These mice were viable and fertile and had no observable health problems up to the age of one year when housed in pathogen-free conditions. These mice were resistant to cardiac pathological remodeling and dysfunction induced by 4-week pressure overload compared to control mice.
    Strain GQC Summary
    Gene Specific Genotyping:

    To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

    Background Genetic Quality:

    The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Apoptosis
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Diabetes
    • Immunology and Inflammation
    • Metabolism
    • Models for Human Disease
    • Neurobiology
    • Obesity
    • Reproduction
    • Research Tools

    Strain Origin

    Submitter
    Taixing Cui, M.D., University of South Carolina.
    Primary Reference

    Strain Development
    Transgenic mice with cardiac myocyte-specific overexpression of UCHL1 (CR-Uchl1 Tg) were produced in a FVB/NJ background using the transgene cassette containing a murine 5.5kb alpha-myosin heavy chain (α-MHC) promoter. Five founders of CR-Uchl1 Tg mice were identified by PCR, 248-B1, 248-D1, 248-F5, 248-I2, and 248-K2. One line with modest overexpression of the inserted human UCHL1 gene was established by backcrossing with wild-type FVB/NJ (JAX Strain #001800) more than 10 times. Mice were then sib-mated for at least 5 generations.

    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    White
    Eye
    Pink

    Breeding

    MMRRC Breeding System
    Backcross then sib-mating
    Generation
    N10+ (FVB/NJ), F5+
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: N/A N/A
    Age Reproductive Decline: 4 to 6 months 7 to 9 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    The submitter or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    066727-UNC-RESUS Litter recovered from cryo-archive $3,566.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.