Loading...

Strain Detail Sheet

Published: 02/19/2020
Revised: 04/21/2020

Strain Name:
FVB/NJ-Tg(Myh6-CYLD)247E3Cuit/Mmnc
Stock Number:
066729-UNC
Citation ID:
RRID:MMRRC_066729-UNC
Other Names:
CR-Cyld-tg

Strain Information

Gene Details

Tg(Myh6-CYLD)247E3Cuit
Name: transgene insertion 247E3, Taixing Cui
Type: Allele
Species: Multi-species
Chromosome: unknown
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 15
CYLD
Name: CYLD lysine 63 deubiquitinase
Type: Gene
Species: Homo sapiens (human)
Chromosome: 16
Genetic Alterations
Randomly inserted transgene construct with human CYLD1 driven by the murine, cardiac Myh6 promoter.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.

    • Strain Description

    Phenotype
    CYLD, a deubiquitinating enzyme, is a critical regulator of diverse cellular processes, ranging from proliferation and differentiation to inflammatory responses. Rapid growing evidence has demonstrated that CYLD plays a critical role in immunity, lipid metabolism, spermatogenesis, osteoclastogenesis antimicrobial defense, and inflammation. The donor's lab has been focusing on CYLD for many years. CR-Cyld-tg mice are easy breeders with standard genotyping; they constitute a useful tool in cardiac disease. These mice were viable and fertile and had no observable health problems up to the age of one year, when housed in pathogen-free conditions. These mice were resistant to cardiac pathological remodeling and dysfunction induced by 4-week pressure overload.
    MeSH Terms
    • Animals
    • Cell Proliferation
    • Cells, Cultured
    • Enzyme Activation
    • Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors
    • Extracellular Signal-Regulated MAP Kinases/biosynthesis
    • Humans
    • Muscle, Smooth, Vascular/cytology
    • Muscle, Smooth, Vascular/drug effects
    • Muscle, Smooth, Vascular/physiology
    • Myocytes, Smooth Muscle/cytology
    • Myocytes, Smooth Muscle/drug effects
    • Myocytes, Smooth Muscle/physiology
    • Protein Biosynthesis
    • Rats
    • Rats, Sprague-Dawley
    • Tumor Necrosis Factor-alpha/metabolism
    • Tumor Necrosis Factor-alpha/pharmacology
    • Ubiquitin Thiolesterase/genetics
    • Ubiquitin Thiolesterase/metabolism
    • Vasculitis/enzymology
    Strain GQC Summary
    Gene Specific Genotyping:

    To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

    Background Genetic Quality:

    The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Apoptosis
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Diabetes
    • Endocrine Deficiency
    • Immunology and Inflammation
    • Metabolism
    • Models for Human Disease
    • Neurobiology
    • Obesity
    • Reproduction
    • Research Tools

    Strain Origin

    Submitter
    Taixing Cui, M.D., University of South Carolina.
    Primary Reference

    Additional References

    Mathis BJ, Lai Y, Qu C, Janicki JS, Cui T. CYLD-mediated signaling anddiseases. Curr Drug Targets. 2015;16(4):284-94. Review. (Medline PMID: 25342597)

    Strain Development

    Transgenic mice with cardiac myocyte-specific overexpression of human CYLD1were produced in an FVB/NJ background using a transgene cassette containing 5.5 kb of the muring alpha-myosin heavy chain, α-MHC, promoter (gene symbol: Myh6). Founder 247 (designating construct, litter, and mouse, respectively) was backcrossed to FVB/NJ (RRID:IMSR_JAX:001800) >10 times. The mice were then sib-mated for at least 5 generations prior to archival at the MMRRC.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    White
    Eye
    Pink

    Breeding

    MMRRC Breeding System
    Backcross then sib-mating
    Generation
    N10+ (FVB/NJ), F5+
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: N/A N/A
    Age Reproductive Decline: 4 to 6 months 7 to 9 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    The submitter or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    066729-UNC-RESUS Litter recovered from cryo-archive $3,566.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.