Strain Detail Sheet

Strain Name:
B6J.B6N(129S4)-Hscbtm1c(EUCOMM)Hmgu/MdfMmjax
Stock Number:
066763-JAX
Citation ID:
RRID:MMRRC_066763-JAX
Other Names:
Hscb cKO

Strain Information

Gene Details

Hscbtm1c(EUCOMM)Hmgu
Name: HscB iron-sulfur cluster co-chaperone; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
Synonyms: Hscbfl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Conditional
Hscb
Name: HscB iron-sulfur cluster co-chaperone
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Conditional
NCBI: 100900
Homologene: 13945
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Additional Resources
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KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Excision of FRT-Neo cassette
Genotype Determination
ES Cell Line
JM8A3.N1 derived from C57BL/6N-Atm1Brd

Strain Description

Phenotype
Homozygous KO animals are not viable. Conditional KO bred to blood/red blood cell-specific CRE transgenic lines develop pancytopenia and sideroblastic anemia. Conditional homozygous mice have no phenotype
Strain Development
Mice carrying the Hscbtm1a allele on B6N background (EuMMCR) were bred to B6N.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J (RRID:IMSR_JAX:016226) to excise the Neo/Lacz cassette. The resulting progeny were backcrossed 20 generations to C57BL/6J (RRID:IMSR_JAX:000664), then sib-mated for about 5 generations.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cell Biology
  • Developmental Biology
  • Hematology
  • Metabolism

Strain Origin

Donor
Mark Fleming, M.D., Boston Children's Hospital.
Primary Reference

Crispin A, Guo C, Chen C, Campagna DR, Schmidt PJ, Lichtenstein DA, Cao C, Sendamarai AK, Hildick-Smith GJ, Huston NC, Boudreaux J, Bottomley SS, Heeney MM, Paw BH, Fleming MD, Ducamp S. Mutations in the iron-sulfur cluster biogenesis protein HSCB cause congenital sideroblastic anemia. J Clin Invest. 2020 Jul 7:135479. doi: 10.1172/JCI135479. Epub ahead of print. (Medline PMID: 32634119)

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross and sib-mating
Generation
N20 (C57BL/6J), F5+
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months Greater than 12 months
Bred to Homozygosity
Yes
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.