Strain Name:
B6J.FVB-Gt(ROSA)26Sortm3(CAG-SPTLC2/SPTSSA/SPTLC1,-EGFP)1Rlp/Mmucd
Stock Number:
066773-UCD
Citation ID:
RRID:MMRRC_066773-UCD
Other Names:
floxed stop fusion-serine palmitoyl transferase

Strain Information

Gt(ROSA)26Sortm3(CAG-SPTLC2/SPTSSA/SPTLC1,-EGFP)1Rlp
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Richard Proia
Type: Allele
Species: Multi-species
Chromosome: 6
CAG
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Type: DNA Segment
Species: Multi-species
Chromosome:
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910
SPTLC2
Name: serine palmitoyltransferase long chain base subunit 2
Type: Gene
Species: Homo sapiens (human)
Chromosome: 14
SPTLC1
Name: serine palmitoyltransferase long chain base subunit 1
Type: Gene
Species: Homo sapiens (human)
Chromosome: 9
SPTSSA
Name: serine palmitoyltransferase small subunit A
Type: Gene
Species: Homo sapiens (human)
Chromosome: 14
Genetic Alterations
The transgene is a single-chain version of serine palmitoyltransferase with its three core (human) subunit cDNAs, SPTLC2, SPTSSA, and SPTLC1 fused into a single reading frame linked T2A-separated EGFP under control of a promoter that is activated by Cre recombinase expression (Stop-fSPT). The transgene was inserted into the Gt(ROSA)26Sor locus using TARGATTTM technology (Applied Stem Cell, Milpitas, CA).
ES Cell Line
C57BL/6N-derived ESC line
Phenotype
Overexpression of the first enzyme of the sphingolipid metabolism pathway. Cre-mediated inducible elevation of sphingolipid synthesis. Per donor, at the time of the archiving, this is the only mouse line that allows inducible expression of the sphingolipid synthesis pathway.
Strain Development
To generate mice to enable the conditional expression of fusion SPT (fSPT), the three individual SPT subunits (in the following order: SPTLC2, SPTSSA, and SPTLC1) were cloned as a single-chain coding unit linked to EGFP by a T2A peptide sequence (Figs. 4 and S1A, PMID:31880535). A stop cassette flanked by LoxP sites was inserted between the CAG promoter and the fSPT coding unit. The expression cassette was inserted into the Rosa26 locus using TARGATT methodology originally developed on FVB. Subsequent backcrossing to C57BL/6J (N7).
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Diabetes
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Obesity
  • Research Tools
Donor
Richard Proia, Ph.D., National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health.
Primary Reference

Clarke BA, Majumder S, Zhu H, Lee YT, Kono M, Li C, Khanna C, Blain H,Schwartz R, Huso VL, Byrnes C, Tuymetova G, Dunn TM, Allende ML, Proia RL. TheOrmdl genes regulate the sphingolipid synthesis pathway to ensure propermyelination and neurologic function in mice. Elife. 2019 Dec 27;8. pii: e51067.doi: 10.7554/eLife.51067. (Medline PMID: 31880535)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Generation
N7 (C57BL/6J)
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
066773-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
066773-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.