Strain Name:
B6J.129X1-Abcc8tm1Jbry/Mmnc
Stock Number:
066787-UNC
Citation ID:
RRID:MMRRC_066787-UNC
Other Names:
Sulfonylurea receptor type 1 knock-out

Strain Information

Abcc8tm1Jbry
Name: ATP-binding cassette, sub-family C member 8; targeted mutation 1, Joseph Bryan
Synonyms: Sur1KO, Sur1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Abcc8
Name: ATP-binding cassette, sub-family C member 8
Synonyms: Sur, SUR1, D930031B21Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Genetic Alterations
Gene disruption by replacement of exon 2 with a puromycin resistance cassette via homologous recombination.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    RW-4 (129X1/SvJ)
    Phenotype
    None/Normal/Wild-type
    MeSH Terms
    • ATP-Binding Cassette Transporters
    • Animals
    • Hypoglycemia
    • Insulin/metabolism
    • Insulin Secretion
    • Islets of Langerhans/metabolism
    • Islets of Langerhans/physiology
    • Membrane Potentials
    • Mice
    • Mice, Knockout
    • Models, Biological
    • Potassium Channels/genetics
    • Potassium Channels/metabolism
    • Potassium Channels/physiology
    • Potassium Channels, Inwardly Rectifying
    • Receptors, Drug/genetics
    • Receptors, Drug/metabolism
    • Sulfonylurea Compounds/metabolism
    • Sulfonylurea Receptors
    • Analgesics/pharmacology
    • Analgesics/therapeutic use
    • Diazoxide/pharmacology
    • Diazoxide/therapeutic use
    • Evoked Potentials
    • Female
    • Hyperalgesia/drug therapy
    • Hyperalgesia/metabolism
    • Male
    • Mice, Inbred C57BL
    • Potassium Channels, Inwardly Rectifying/metabolism
    • Spinal Nerves/drug effects
    • Spinal Nerves/metabolism
    • Spinal Nerves/physiopathology
    • Sulfonylurea Receptors/agonists
    • Sulfonylurea Receptors/genetics
    • Sulfonylurea Receptors/metabolism
    • Touch
    Strain Development
    The targeting vector flanked by a puromycin resistance cassette was transfected into RW-4 embryonic stem cells by electroporation. Puromycin-resistant clones were isolated and screened by Southern blotting to identify homologous recombinants. The selected embryonic stem cells were injected into C57BL/6 blastocysts to generate germline chimeras. Chimeric males were then selected and crossed with wild type C57BL/6 females to generate Sur1+/– heterozygotes, which were bred to obtain Sur1+/+, Sur1+/–, and Sur1–/– animals. Subsequent backcrossing of the homozygous KOs to C57BL/6J for >10 generations.
    Suggested Control Mice
    C57BL/6J
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cardiovascular
    • Diabetes
    • Neurobiology
    Donor
    Joseph Bryan, Ph.D., Pacific Northwest Diabetes Research Institute.
    Primary Reference

    Seghers V, Nakazaki M, DeMayo F, Aguilar-Bryan L, Bryan J. Sur1 knockout mice.A model for K(ATP) channel-independent regulation of insulin secretion. J BiolChem. 2000 Mar 31;275(13):9270-7. (Medline PMID: 10734066)

    Fisher C, Johnson K, Okerman T, Jurgenson T, Nickell A, Salo E, Moore M,Doucette A, Bjork J, Klein AH. Morphine Efficacy, Tolerance, and HypersensitivityAre Altered After Modulation of SUR1 Subtype K(ATP) Channel Activity in Mice.Front Neurosci. 2019 Oct 22;13:1122. doi: 10.3389/fnins.2019.01122. eCollection2019. (Medline PMID: 31695594)

    Luu W, Bjork J, Salo E, Entenmann N, Jurgenson T, Fisher C, Klein AH.Modulation of SUR1 K(ATP) Channel Subunit Activity in the Peripheral NervousSystem Reduces Mechanical Hyperalgesia after Nerve Injury in Mice. Int J Mol Sci.2019 May 7;20(9). pii: E2251. doi: 10.3390/ijms20092251. (Medline PMID: 31067750)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross and sib-mating
    Generation
    N10+ (C57BL/6J)
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Bred to Homozygosity
    Yes
    Average litter size
    4 to 6
    Recommended wean age
    3 weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    066787-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.