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Availability & Fees Order this Strain
Genotyping Protocol
Conditional phenotype: The effects of Kdm6a knockout are described in PMID:23028370, although on a different allele. Male and female mice with one copy of the floxed allele breed as well as wild-type C57BL/6J. XX females with complete KO of Kdm6a die at mid-gestation because of cardiac and other abnormalities. In the donor's laboratory, heterozygous females (+/-) breed, but have small litters. Males (-/y) with one KO allele are small. Note that fertility data below refer to the floxed allele, not the KO.
Kdm6a is a histone demethylase gene with widespread effects that are just being discovered. Kdm6a is X-linked and expressed constitutively higher in XX than XY cells, so it causes sex differences in disease phenotypes. This donation is part of an integrated set of mouse resources that have been donated to the MMRRC from the Arnold lab, very useful for uncovering sex-specific protective mechanisms of disease. The lab of Sean Li at Harvard used a related mouse line to show that Kdm6a dose influences mortality, and explains some of the sex differences, in a model of bladder cancer (PMID:29928692).
Itoh Y, Golden LC, Itoh N, Matsukawa MA, Ren E, Tse V, Arnold AP, Voskuhl RR. The X-linked histone demethylase Kdm6a in CD4+ T lymphocytes modulatesautoimmunity. J Clin Invest. 2019 Aug 12;130:3852-3863. doi: 10.1172/JCI126250.eCollection 2019 Aug 12. (Medline PMID: 31403472)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.