Strain Name:
B6N(129S4)-Setbp1tm1c(EUCOMM)Wtsi/LutzyMmjax
Stock Number:
066873-JAX
Citation ID:
RRID:MMRRC_066873-JAX
Other Names:
B6N(129S4)-Setbp1tm1c(EUCOMM)Wtsi/IcsOrlMmjax

Gene Information

Setbp1tm1c(EUCOMM)Wtsi
Name: SET binding protein 1; targeted mutation 1c, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Conditional
Setbp1
Name: SET binding protein 1
Synonyms: Seb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Conditional
NCBI: 240427
Homologene: 9192
Genetic Alterations
C57BL/6N-derived JM8A1.N1 embryonic stem (ES) cells (clone EPD0445_3_E05) bearing the Setbp1tm1a(EUCOMM)Wtsi-targeted allele were obtained from The European Conditional Mouse Mutagenesis Program (EUCOMM). This construct incorporates an FRT-lacZ-loxP followed by a neomycin resistance cassette, SV40 polyA, a second FRT site and a second loxP site in intron 3. A third loxP site is inserted downstream in intron 5 (position 78855613). The FRT sites were modified with an Flp-expressing line.
Genotype Determination
ES Cell Line
JM8A3.N1 derived from C57BL/6N
Phenotype
Unknown, but expected to be normal. Conditional phenotype unknown.
Strain Development
Targeted ES cells were injected into blastocysts. The mice were bred to transgenic mice expressing the flp recombinase (B6N.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J - JAX Strain #016226), to excise the FRT site-flanked lacZ and neo cassette leaving exon 4 flanked by loxP sites. Mice were backcrossed to C57BL/6NJ (JAX Strain #005304).
Suggested Control Mice
C57BL/6N
  • Models for Human Disease
  • Neurobiology
Donor
Cat Lutz, Ph.D., The Jackson Laboratory.

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Generation
N1 (C57BL/6NJ), F2
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
066873-JAX-HOM-F
066873-JAX-HOM-M
Homozygous Female
Homozygous Male
$218.00 / $218.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
066873-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.