Loading...

Strain Detail Sheet

Published: 05/06/2020
Revised: 03/01/2026

Strain Name:
B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Hprt1tm2(tetO-mediumFT*)Sguo/Mmjax
Stock Number:
066959-JAX
Citation ID:
RRID:MMRRC_066959-JAX
Other Names:
iH2B-FT-Medium

Strain Information

Gene Details

Gt(ROSA)26Sortm1(rtTA*M2)Jae
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rudolf Jaenisch
Synonyms: Rosa26-M2rtTA, R26-M2rtTA, R26-rtTA, Gt(ROSA)26Sortm1(M2rtTA)Jae, M2-rtTA, M2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
Hprt1tm2(tetO-mediumFT*)Sguo
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Shangqin Guo
Synonyms: iH2B-FT-Medium, Hprttm2(tetO/CMV-H2BC9/mediumFT)Sguo, HPRT::iH2B-FT-Medium
Type: Allele
Species: Multi-species
Chromosome: X
Alteration at locus: Knock-In
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Hprt1
Name: hypoxanthine phosphoribosyltransferase 1
Synonyms: Hprt1, Hprt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 15452
HGNC: HGNC:5157
Homologene: 56590

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
mCherry
Name: red fluorescent fluorophore, mCherry
Type: Gene
Species: Discosoma sp. (mushroom coral)
Chromosome:
rtTA*M2
Name: reverse tetracycline controlled transactivator, mutant #2
Type: Gene
Species: Mus musculus (mouse)
Chromosome:
H2BC9
Name: H2B clustered histone 9
Type: Gene
Species: Homo sapiens (human)
Chromosome: 6
Genetic Alterations
This is a novel reporter mouse expressing a fluorescent timer protein (FT-Medium) fused to histone H2B (gene: H2BC9). H2B-FT-Medium is under the control of a doxycycline-inducible promoter (TetO) and knocked into the mouse genome at the HPRT locus on the X chromosome. The mouse also has an rtTA transgene knocked into the Rosa26 locus. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA, predicting amino acid exchanges S12G, E19G, A56P, D148E and H179R. Mice are homozygous for the rtTA allele and either homozygous (females) or hemizygous (males) for the iH2B-FT-Medium allele.

Compared to mCherry (RefSeq GenBank AHW57114.1), FT-medium has genetic alterations predicting the following amino acid substitutions: M23E, N28D, T48S, K75R, L88W, M155I, Q193L, L204M, Y219C, R225H.
Genotype Determination
ES Cell Line
  • Hprttm2(tetO/CMV-H2B/mediumFT)Sguo allele - A2Lox.cre derived from 129P2/OlaHsd
  • Gt(ROSA)26Sortm1(rtTA*M2)Jae allele - KH2 derived from (C57BL/6 x 129S4/SvJae)F1

Strain Description

Phenotype
None/Normal/Wild-type.

Conditional phenotype: The mouse has whole-body expression of a fluorescent fusion reporter, TetO-H2B-FT-Medium, when induced with doxycycline drinking water.

Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Neurobiology
  • Research Tools

Strain Origin

Submitter
Shangqin Guo, Ph.D., Yale University.
Primary Reference

Resolving cell cycle speed in one snapshot with a live-cell fluorescent reporter, Anna E. Eastman, Xinyue Chen, Xiao Hu, Amaleah A. Hartman, Aria M. Pearlman Morales, Cindy Yang, Jun Lu, Hao Yuan Kueh, Shangqin Guo, bioRxiv 494252; doi: https://doi.org/10.1101/494252

Strain Development

Correctly targeted blastocysts were implanted into C57BL/6 females. High-degree chimeric male offspring were selected by coat color and backcrossed onto C57BL/6J to N6+. The rtTA allele was introduced by cross-breeding with a strain on C57BL/6J background. Subsequent SNP analysis revealed incomplete backcrossing; the strains genetic background is, hence, a mix of C57BL/6, 129S4, and 129P2.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcrossing and random intra-strain mating
Generation
N6+ (C57BL/6J)
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes N/A
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
Variable
Recommended wean age
3 Weeks
Average Pups Weaned
Variable

Order Information

Availability Level

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

The submitter or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.