Strain Detail Sheet

Strain Name:
B6(129S4)-Tg(tetO-Cxcl12*,-tdTomato)#Forsb/Mmjax
Stock Number:
067169-JAX
Citation ID:
RRID:MMRRC_067169-JAX
Other Names:
Tg-CXCL12(OE)

Strain Information

Gene Details

Tg(tetO-Cxcl12*,-tdTomato)#Forsb
Name: transgen insertion #, Camilla Forsberg
Type: Allele
Species: Multi-species
Chromosome: unknown
Cxcl12
Name: C-X-C motif chemokine ligand 12
Synonyms: TLSF-a, Sdf1, Scyb12, SDF-1, TPAR1, TLSF-b, PBSF/SDF-1, Sdf1a, Sdf1b, PBSF
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20315
Homologene: 128606
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tdTomato
Name:
Type: Gene
Species: Discosoma sp. (mushroom coral)
Chromosome:
tetO
Name: tetracycline-responsive promoter element
Type: Regulatory Region
Species: Multi-species
Chromosome:
Genetic Alterations
Random insertion of a tetO-driven transgene construct comprising Cxcl12 and tdTomato, separated from Cxcl12 by a 2A self-cleaving peptide.
Genotype Determination

Strain Description

Phenotype
Transgenic overexpression of murine Cxcl12 and tdTomato under the control of Tet(O) promoter by doxycycline. CXCL12 is secreted but tdTomato is membrane-bound to the cell that transcribes the gene. Crossing this strain with specific Tet(ON) strains will allow specific overexpression.

Conditional phenotype: Only overexpressed by crossing with an rtTA line (e.g., MMRRC:14112); upon addition of doxycycline (by chow or water)

Strain Development

The transgenic construct "pRP(Exp)-TRE3G>6xHis/CXCL12/T2A/tdTomato" was injected into C57BL/6J mice, and 7 founders were identified. The founders were backcrossed once to C57BL/6J mated with B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J mice (RRID:IMSR_JAX:006965). The progeny were then sibling-crossed or crossed between different founders and screened for the presence of the transgene, for expansion of the colony. Prior to archival at the MMRRC, the rtTA allele was bred out with C57BL/6J.

Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor
E. Camilla Forsberg, Ph.D., UC Santa Cruz.
Primary Reference

Rajendiran S, Smith-Berdan S, Kunz L, Risolino M, Selleri L, Schroeder T, Forsberg EC. Ubiquitous overexpression of CXCL12 confers radiation protection and enhances mobilization of hematopoietic stem and progenitor cells. Stem Cells. 2020 May 22. doi: 10.1002/stem.3205. Epub ahead of print. (Medline PMID: 32442338)

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross and random intra-strain mating
Generation
N3+ (C57BL/6J)
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: N/A N/A
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
Between 3 and 4 weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.