Strain Name:
STOCK Suv39h2em1Ksz Suv39h1em1Ksz/Mmjax
Stock Number:
067176-JAX
Citation ID:
RRID:MMRRC_067176-JAX
Other Names:
Suv39h1; Suv39h2 double mutant

Strain Information

Suv39h2em1Ksz
Name: suppressor of variegation 3-9 2; endonuclease-mediated mutation 1, Kenneth Zaret
Synonyms: Suv39h2em1Jnw
Type: Allele
Species: Mus musculus (mouse)
Chromosome: Chr 10
Alteration at locus: CRISPR
Suv39h1em1Ksz
Name: suppressor of variegation 3-9 1; endonuclease-mediated mutation 1, Kenneth Zaret
Synonyms: Suv39h1fl, Suv39h1em1Jnw
Type: Allele
Species: Mus musculus (mouse)
Chromosome: Chr 2
Alteration at locus: CRISPR
Suv39h2
Name: suppressor of variegation 3-9 2
Synonyms: 4930507K23Rik, Suv39h histone methyltransferase, D2Ertd544e, KMT1B
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: CRISPR
NCBI: 64707
Homologene: 32548
Suv39h1
Name: suppressor of variegation 3-9 1
Synonyms: mIS6, DXHXS7466e, Suv39h histone methyltransferase, KMT1A
Type: Gene
Species: Mouse
Chromosome: X
Alteration at locus: CRISPR
NCBI: 20937
Homologene: 2388
Genetic Alterations
The Suv39h1 gene has been floxed by insertion of or LoxP sites into intron 2 and 5. The Suv39h2 has been knocked out from both copies through CRISPR-targeting of exon 2 producing a 4-bp deletion frameshift null allele.

HGVS nomenclature (Suv39h2 variant):

  • Genbank RefSeq - mRNA: NM_022724.4
  • Genbank RefSeq, protein: NP_073561.2
  • Variant, nucleic acid level: c.320_323del
  • Variant, amino acid level, predicted: p.Ser107Leufs*8
    Check this variant: LUMC Mutalyzer
ES Cell Line
Not applicable
Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • Cell Differentiation
  • Cell Lineage
  • Embryo, Mammalian
  • Embryonic Stem Cells/cytology
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Silencing
  • Germ Layers/cytology
  • Hepatocytes/cytology
  • Heterochromatin/genetics
  • Histones/chemistry
  • Insulin-Secreting Cells/cytology
  • Methylation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Organogenesis
Strain Development
CRISPR/Cas9 technology was used to insert loxP sites into Suv39h1 introns using B6C3F1 zygotes, and to direct the Cas9 to exon 2 in Suv39h2 gene. Positive pups were intercrossed to obtain homozygous mice for the two genes.
Suggested Control Mice
Suv39h1flox/flox; Suv39h2mut/wt
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation
  • Metabolism
  • Obesity
Donor
Ken Zaret, Ph.D., University of Pennsylvania, Perelman School of Medicine.
Primary Reference

Nicetto D, Donahue G, Jain T, Peng T, Sidoli S, Sheng L, Montavon T, Becker JS, Grindheim JM, Blahnik K, Garcia BA, Tan K, Bonasio R, Jenuwein T, Zaret KS. H3K9me3-heterochromatin loss at protein-coding genes enables developmental lineage specification. Science. 2019 Jan 18;363(6424):294-297. doi: 10.1126/science.aau0583. Epub 2019 Jan 3. (Medline PMID: 30606806)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
N/A
Overall Breeding Performance
Undetermined
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Bred to Homozygosity
Yes
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Viviane Martin.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Viviane Martin

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067176-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
067176-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.