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Myo7a Conditional Ready (tm1c)
These mice are conditional-ready tm1c mice, Flp-modified from the original Myo7atm1a(EUCOMM)Wtsi allele. They have also been backcrossed onto a C57BL/6J background and are now free of the Crb1rd8 retinal degeneration allele (MGI:2676366) inherent to the original C57BL/6N background.
The L1L2_Bact_P cassette was inserted at position 98095169 of Chromosome 7 upstream of the critical exon 10 (Build GRCm38). This initial cassette was composed of an FRT site followed by a lacZ sequence and a loxP site. The first loxP site was followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site, and a second loxP site. A third loxP site was inserted downstream of exon 11 at position 98093985. Critical exons 10 and 11 were thus flanked by loxP sites.
These mice do not display any abnormal phenotypes. Prior to archival at the MMRRC, they were maintained through homozygous x homozygous breeding. There were no issues with the viability/fertility. Importantly, this line has been confirmed to be free of the C57BL/6N-associated Crb1rd8 allele, which, in the homozygous state, leads to retinal degeneration. All mice in KOMP were made on the C57BL/6N background. This has confounded many studies of gene function in the retina.
These mice were generated as part of the Sanger Mouse Genetics Project (PMID:23912999). Mouse ES cells were created as part of the International Knockout Mouse Consortium and were acquired from the Sanger Institute. The knockout-first allele (tm1a) was generated using JM8A3.N1 (C57BL/6N) ES cells. ES cells were implanted in C57BL/6N mice. Resulting tm1a mice were backcrossed on the C57BL/6J background and screened to ensure the removal of the Crb1rd8 allele. Resultant weans were crossed with B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ (RRID:IMSR_JAX:009086) to create the Myo7a conditional-ready (tm1c) strain. These mice have been backcrossed minimally twice to C57BL/6J and sib-mated to F13.
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A. A conditional knockout resource for the genome-wide study of mouse gene function. Nature. 2011 Jun 15;474(7351):337-42. doi: 10.1038/nature10163. (Medline PMID: 21677750)
Ryder E, Gleeson D, Sethi D, Vyas S, Miklejewska E, Dalvi P, Habib B, Cook R, Hardy M, Jhaveri K, Bottomley J, Wardle-Jones H, Bussell JN, Houghton R, Salisbury J, Skarnes WC; Sanger Mouse Genetics Project, Ramirez-Solis R. Molecular characterization of mutant mouse strains generated from the EUCOMM/KOMP-CSD ES cell resource. Mamm Genome. 2013 Aug;24(7-8):286-94. doi: 10.1007/s00335-013-9467-x. Epub 2013 Aug 4. (Medline PMID: 23912999)
Colony Surveillance Program and Current Health Reports
Backcross and sib-mating
N2+ (C57BL/6J), F13
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.