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Availability & Fees Order this Strain
Genotyping Protocol
The targeting vector (Ai9 - see RRID:IMSR_JAX:007909) was validated by Sanger sequencing and used to target C57BL/6 embryonic stem cells with the assistance of the University of Colorado Bioengineering Core facility. After selection for neomycin resistance, several properly-targeted clones were obtained, and two were karyotyped. One normal karyotyped embryonic stem cell line was then used to generate chimeras, which were backcrossed twice to C57BL/6J. This was followed by maintenance via intercrossing heterozygous mice. The line was bred to 129S1.Cg-Tg(Vsx2-cre)2690Chow/J (RRID:IMSR_JAX:26200; originally made on a mixed C57BL/6;C3H background). After a few crosses, the ROSA-Prdm1 line was bred out from the Vsx2-Cre via a backcross to C57BL/6J (i.e., the LSL is intact). At this point (2018), the line was once again maintained by sib-mating. Prior to arrival at the MMRRC, the line was backcrossed to C57BL/6J (N<5).
Goodson NB, Park KU, Silver JS, Chiodo VA, Hauswirth WW, Brzezinski JA 4th. Prdm1 overexpression causes a photoreceptor fate-shift in nascent, but not mature, bipolar cells. Dev Biol. 2020 Aug 15;464(2):111-123. doi: 10.1016/j.ydbio.2020.06.003. Epub 2020 Jun 17. (Medline PMID: 32562755)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Deborah Bernard.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Deborah Bernard
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.