Strain Name:
C57BL/6N-Slc13a5em2Jcch/Mmucd
Stock Number:
067434-UCD
Citation ID:
RRID:MMRRC_067434-UCD
Other Names:
B6-Slc13a519del, C57BL/6N-Slc13a5em2Jcch/Mmnc

Gene Information

Slc13a5em2Jcch
Name: solute carrier family 13 (sodium-dependent citrate transporter), member 5; endonuclease-mediated mutation 2, Jan Hu
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: CRISPR
Slc13a5
Name: solute carrier family 13 (sodium-dependent citrate transporter), member 5
Synonyms: Nact, NaC2/NaCT, mINDY, Indy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: CRISPR
NCBI: 237831
Homologene: 21941
Genetic Alterations
Solute carrier family 13 (sodium-dependent citrate transporter), member 5 (Gene Synonyms: Indy, NaC2/NaCT, Nact, mINDY). Chromosome 11: 72,241,989-72,267,222 reverse strand. GRCm38:CM001004.2 sgRNA and Cas9 were used to induce a double-strand chromosome break in exon 7 near codon 337. A single-stranded oligonucleotide donor to change codon 337 from Arg (CGA) to Opal (TGA) was also injected. In the editing process, a deletion of 19 nucleotides occurred, NM_001004148.4:c.1685_1703delCCGACTGGGCAAATTCAAC

HGVS nomenclature:

  • Genbank RefSeq - mRNA: NM_001004148.4
  • Genbank RefSeq, protein: NP_001004148.1
  • Variant, nucleic acid level: c.1685_1703delCCGACTGGGCAAATTCAAC
  • Variant, amino acid level, predicted: p.Pro562Glnfs*22
    Check this variant: LUMC Mutalyzer
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    There are no perceivable differences in growth, development, physical activities, reproduction, and life span between Slc13a519del mutants and wild-type mice. The Slc13a519del mice have thin and chalky white enamel mimicking hypoplastic-hypomaturation amelogenesis imperfecta in human (EIEE25).
    Strain Development
    Crossed with C57BL/6N wild-type for 2 generations then sib-pair breeding
    Suggested Control Mice
    Wild-type littermates
    • Models for Human Disease
    Donor
    Jan Ching Chun Hu, Ph.D., University of Michigan.
    Primary Reference
    Not ready to publish

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross and sib-mating
    Generation
    N2 (C57BL/6N), F6
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 4 to 6 months 4 to 6 months
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    Less than 4

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    067434-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    067434-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $7,533.58
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.