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Strain Name:
C57BL/6J-MtgxR8017Btlr/Mmmh
Stock Number:
067457-MU
Citation ID:
RRID:MMRRC_067457-MU
Other Names:
R8017 (G1)
Major Collection:

Strain Information

Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Elp2
Name: elongator acetyltransferase complex subunit 2
Synonyms: Stat3-interacting protein, StIP1, Statip1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 58523
VEGA: 18
Homologene: 6019
Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752
Bcl2l2
Name: BCL2-like 2
Synonyms: bclw, Gtrgal2, Gtrosa41, Bcl-w
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12050
HGNC: HGNC:995
Homologene: 2989
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Bnc2
Name: basonuclin zinc finger protein 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242509
Homologene: 18243
Ppp2r5e
Name: protein phosphatase 2, regulatory subunit B', epsilon
Synonyms: protein phosphatase 2A subunit beta, 4633401M22Rik, B56beta
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26932
VEGA: 12
HGNC: HGNC:9313
Homologene: 55962
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,612,766 bp
  • C to T, chromosome 1 at 121,448,264 bp
  • T to C, chromosome 1 at 192,846,521 bp
  • C to T, chromosome 2 at 76,767,457 bp
  • T to A, chromosome 2 at 87,747,048 bp
  • T to A, chromosome 2 at 89,303,251 bp
  • A to C, chromosome 2 at 90,138,826 bp
  • A to C, chromosome 2 at 111,622,067 bp
  • G to A, chromosome 2 at 111,960,573 bp
  • T to G, chromosome 2 at 156,555,956 bp
  • T to A, chromosome 2 at 164,333,421 bp
  • T to C, chromosome 3 at 129,855,099 bp
  • T to C, chromosome 4 at 8,604,444 bp
  • T to C, chromosome 4 at 42,850,840 bp
  • A to T, chromosome 4 at 43,034,413 bp
  • G to T, chromosome 4 at 58,146,637 bp
  • A to G, chromosome 4 at 84,411,425 bp
  • A to G, chromosome 4 at 99,986,678 bp
  • G to T, chromosome 4 at 124,850,676 bp
  • G to A, chromosome 4 at 129,428,445 bp
  • T to A, chromosome 4 at 134,073,173 bp
  • A to G, chromosome 4 at 145,622,568 bp
  • C to T, chromosome 5 at 65,073,861 bp
  • T to A, chromosome 5 at 108,228,645 bp
  • A to G, chromosome 5 at 124,800,885 bp
  • C to A, chromosome 5 at 137,612,811 bp
  • T to C, chromosome 5 at 138,301,203 bp
  • A to G, chromosome 6 at 8,658,286 bp
  • C to T, chromosome 6 at 128,581,447 bp
  • A to G, chromosome 6 at 145,194,557 bp
  • A to T, chromosome 7 at 3,841,697 bp
  • T to C, chromosome 7 at 12,989,402 bp
  • T to C, chromosome 7 at 15,397,049 bp
  • T to A, chromosome 7 at 25,080,670 bp
  • T to C, chromosome 7 at 25,748,464 bp
  • A to T, chromosome 7 at 96,704,041 bp
  • C to A, chromosome 7 at 127,108,824 bp
  • T to A, chromosome 8 at 40,754,087 bp
  • A to C, chromosome 8 at 54,638,368 bp
  • T to C, chromosome 8 at 104,616,267 bp
  • T to C, chromosome 9 at 44,799,601 bp
  • G to A, chromosome 9 at 53,373,452 bp
  • G to T, chromosome 9 at 63,339,121 bp
  • G to T, chromosome 9 at 78,707,546 bp
  • C to T, chromosome 9 at 102,723,769 bp
  • C to T, chromosome 9 at 110,602,187 bp
  • A to G, chromosome 9 at 123,637,852 bp
  • T to A, chromosome 9 at 123,664,574 bp
  • A to T, chromosome 10 at 20,354,154 bp
  • A to C, chromosome 10 at 33,909,459 bp
  • C to T, chromosome 10 at 45,638,382 bp
  • T to A, chromosome 10 at 82,060,359 bp
  • G to A, chromosome 10 at 97,483,535 bp
  • C to T, chromosome 11 at 6,618,517 bp
  • A to G, chromosome 11 at 55,164,269 bp
  • G to T, chromosome 11 at 85,914,160 bp
  • T to C, chromosome 11 at 100,374,197 bp
  • G to T, chromosome 11 at 101,124,028 bp
  • A to G, chromosome 11 at 102,843,348 bp
  • G to A, chromosome 12 at 54,044,679 bp
  • A to G, chromosome 12 at 75,464,929 bp
  • C to T, chromosome 12 at 108,681,524 bp
  • A to T, chromosome 12 at 113,702,172 bp
  • G to A, chromosome 13 at 8,887,938 bp
  • G to A, chromosome 13 at 21,574,478 bp
  • T to C, chromosome 13 at 42,167,622 bp
  • C to T, chromosome 13 at 69,627,645 bp
  • T to C, chromosome 13 at 73,799,720 bp
  • T to A, chromosome 13 at 113,319,623 bp
  • G to T, chromosome 14 at 54,638,632 bp
  • A to C, chromosome 14 at 54,858,232 bp
  • T to A, chromosome 14 at 54,884,383 bp
  • T to G, chromosome 14 at 118,267,539 bp
  • T to A, chromosome 15 at 9,172,230 bp
  • T to C, chromosome 15 at 12,373,036 bp
  • C to T, chromosome 15 at 54,254,202 bp
  • A to G, chromosome 15 at 55,064,617 bp
  • T to A, chromosome 15 at 63,826,913 bp
  • A to G, chromosome 15 at 101,693,869 bp
  • T to A, chromosome 15 at 101,920,287 bp
  • G to A, chromosome 16 at 23,357,835 bp
  • A to G, chromosome 16 at 31,007,819 bp
  • G to A, chromosome 17 at 28,550,728 bp
  • G to A, chromosome 18 at 24,606,863 bp
  • A to G, chromosome 18 at 37,727,730 bp
  • G to T, chromosome 18 at 68,235,669 bp
  • T to C, chromosome 19 at 5,082,560 bp
  • T to C, chromosome 19 at 5,111,839 bp
  • T to A, chromosome 19 at 5,477,680 bp
  • TGCGA to T, chromosome 19 at 25,411,204 bp
  • GCGAACG to GCG, chromosome 19 at 25,411,205 bp
  • T to C, chromosome 19 at 34,939,879 bp
  • A to T, chromosome 19 at 45,773,668 bp
  • A to G, chromosome 19 at 55,268,796 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8017 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067457-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.