Strain Name:
C57BL/6J-MtgxR8020Btlr/Mmmh
Stock Number:
067459-MU
Citation ID:
RRID:MMRRC_067459-MU
Other Names:
R8020 (G1)
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: urehr3, Nkcc2, mBSC1, D630042G03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752
Prrx1
Name: paired related homeobox 1
Synonyms: A230024N07Rik, mHox, Prx1, mHox, MHox1, Pmx1, K-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18933
HGNC: HGNC:9142
Homologene: 7896
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Prpf6
Name: pre-mRNA splicing factor 6
Synonyms: ANT-1, 2610031L17Rik, 1190003A07Rik, U5-102K
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68879
Homologene: 5368
Mtif3
Name: mitochondrial translational initiation factor 3
Synonyms: 2810012L14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76366
Homologene: 49927
Rfc1
Name: replication factor C (activator 1) 1
Synonyms: Alp145, 140kDa, RFC140, Recc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Tbc1d14
Name: TBC1 domain family, member 14
Synonyms: 2810413P16Rik, D5Ertd110e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100855
Homologene: 10809
Rplp0
Name: ribosomal protein lateral stalk subunit P0
Synonyms: 36B4, Arbp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11837
Homologene: 6517
Zfp981
Name: zinc finger protein 981
Synonyms: Gm13247
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100041433
Homologene: 133076
Kank1
Name: KN motif and ankyrin repeat domains 1
Synonyms: A930031B09Rik, D330024H06Rik, Ankrd15
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107351
Homologene: 17706
Ms4a6d
Name: membrane-spanning 4-domains, subfamily A, member 6D
Synonyms: Ms4a11
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 68774
Homologene: 130755
Atp8b1
Name: ATPase, class I, type 8B, member 1
Synonyms: Ic, FIC1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 54670
VEGA: 18
HGNC: HGNC:3706
Homologene: 21151
Ighd
Name: immunoglobulin heavy constant delta
Synonyms: IgD
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 380797
HGNC: HGNC:5480
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Hyal4
Name: hyaluronoglucosaminidase 4
Synonyms: 4632428M18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 77042
HGNC: HGNC:5323
Homologene: 69117
Ldlrad4
Name: low density lipoprotein receptor class A domain containing 4
Synonyms: 8230401C20Rik, D330030L18Rik, D18Ertd653e, A430108L08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 52662
VEGA: 18
HGNC: HGNC:1224
Homologene: 3202
Sis
Name: sucrase isomaltase
Synonyms: sucrase-isomaltase, 2010204N08Rik, Si-s
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69983
Homologene: 37424
Dao
Name: D-amino acid oxidase
Synonyms: Dao1, Dao-1, DAO
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13142
HGNC: HGNC:2671
Homologene: 37553
Atxn7l1
Name: ataxin 7-like 1
Synonyms: Atxn7l4, 2810423G08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 380753
Homologene: 53366
A1cf
Name: APOBEC1 complementation factor
Synonyms: apobec-1 complementation factor, 1810073H04Rik, ACF
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 69865
VEGA: 19
Homologene: 16363
Lrrc27
Name: leucine rich repeat containing 27
Synonyms: 2310044E02Rik, 1700071K18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76612
Homologene: 19268
Slc30a9
Name: solute carrier family 30 (zinc transporter), member 9
Synonyms: 2310024J23Rik, GAC63
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 109108
HGNC: HGNC:1329
Homologene: 4627
Il2rb
Name: interleukin 2 receptor, beta chain
Synonyms: Il-2Rbeta, CD122, IL15Rbeta, IL-2/15Rbeta, IL-15 receptor beta chain, IL-15Rbeta
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16185
VEGA: 15
HGNC: HGNC:6009
Homologene: 47955
Gje1
Name: gap junction protein, epsilon 1
Synonyms: Gjf1, AEY12, D230044M03Rik, Gsfaey12, connexin 23, Cx23
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76743
Homologene: 87405
Clpsl2
Name: colipase-like 2
Synonyms: Gm749, LOC328788
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328788
Homologene: 86835
Or51a8
Name: olfactory receptor family 51 subfamily A member 8
Synonyms: GA_x6K02T2PBJ9-5611412-5612350, Olfr570, MOR8-3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259114
Homologene: 128172
Slc35d2
Name: solute carrier family 35, member D2
Synonyms: 5730408I21Rik, hfrc, SQV7L, UGTrel8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70484
VEGA: 13
Homologene: 68119
Gng11
Name: guanine nucleotide binding protein (G protein), gamma 11
Synonyms: 0610037B21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66066
HGNC: HGNC:4403
Homologene: 3045
Prm3
Name: protamine 3
Synonyms: Pxg, PX, Pxg
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19120
Homologene: 74292
Tmem186
Name: transmembrane protein 186
Synonyms: 4432406C05Rik, 2810440M13Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66690
Homologene: 9129
Tsga8
Name: testis specific gene A8
Synonyms: Halap-X, Halapx
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 100502723
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 163,248,262 bp
  • A to T, chromosome 2 at 13,479,178 bp
  • T to C, chromosome 2 at 125,178,102 bp
  • T to C, chromosome 2 at 181,645,570 bp
  • A to G, chromosome 3 at 72,908,965 bp
  • A to G, chromosome 4 at 146,537,368 bp
  • G to T, chromosome 5 at 36,571,843 bp
  • A to T, chromosome 5 at 65,272,178 bp
  • G to T, chromosome 5 at 67,307,033 bp
  • AGG to AG, chromosome 5 at 114,015,209 bp
  • T to C, chromosome 5 at 115,560,844 bp
  • A to G, chromosome 5 at 146,958,903 bp
  • C to T, chromosome 6 at 4,008,077 bp
  • T to C, chromosome 6 at 24,755,996 bp
  • A to G, chromosome 7 at 102,901,265 bp
  • C to T, chromosome 7 at 139,236,877 bp
  • T to C, chromosome 10 at 14,717,277 bp
  • C to T, chromosome 12 at 33,325,953 bp
  • C to T, chromosome 12 at 108,681,524 bp
  • A to T, chromosome 12 at 113,414,548 bp
  • A to G, chromosome 13 at 64,107,043 bp
  • G to A, chromosome 14 at 30,609,244 bp
  • T to C, chromosome 15 at 78,485,004 bp
  • G to A, chromosome 16 at 8,636,160 bp
  • CTCTTCTTCTTCTTC to CTCTTCTTCTTC, chromosome 16 at 10,790,701 bp
  • G to A, chromosome 17 at 28,550,728 bp
  • A to G, chromosome 18 at 64,546,013 bp
  • G to T, chromosome 18 at 68,235,669 bp
  • T to C, chromosome 19 at 11,590,108 bp
  • GCGAACG to GCG, chromosome 19 at 25,411,205 bp
  • T to C, chromosome 19 at 31,893,194 bp
  • A to G, chromosome X at 83,487,098 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8020 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067459-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.