Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8021Btlr/Mmmh
Stock Number:
067460-MU
Citation ID:
RRID:MMRRC_067460-MU
Other Names:
R8021 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Rgs14
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 51791
HGNC: HGNC:9996
Homologene: 4735
Secisbp2
Name: SECIS binding protein 2
Synonyms: 2210413N07Rik, SBP2, 2810012K13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75420
Homologene: 11415
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Sephs1
Name: selenophosphate synthetase 1
Synonyms: SPS1, 1110046B24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109079
Homologene: 56558
Armh3
Name: armadillo-like helical domain containing 3
Synonyms: 9130011E15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71617
VEGA: 19
Homologene: 15843
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 43,554,260 bp
  • T to C, chromosome 1 at 173,759,353 bp
  • T to A, chromosome 1 at 185,322,457 bp
  • T to A, chromosome 2 at 4,906,623 bp
  • T to C, chromosome 2 at 10,105,652 bp
  • A to T, chromosome 2 at 28,686,889 bp
  • T to C, chromosome 2 at 32,259,917 bp
  • A to T, chromosome 2 at 85,430,652 bp
  • A to T, chromosome 2 at 92,386,415 bp
  • T to A, chromosome 2 at 156,022,591 bp
  • T to C, chromosome 2 at 173,209,210 bp
  • T to A, chromosome 3 at 33,809,121 bp
  • T to C, chromosome 3 at 95,747,514 bp
  • T to C, chromosome 3 at 103,928,582 bp
  • T to C, chromosome 4 at 83,459,955 bp
  • T to C, chromosome 4 at 118,968,477 bp
  • T to C, chromosome 4 at 119,352,679 bp
  • G to T, chromosome 4 at 145,148,675 bp
  • A to C, chromosome 5 at 14,539,784 bp
  • C to G, chromosome 5 at 25,287,119 bp
  • T to A, chromosome 5 at 48,302,492 bp
  • T to A, chromosome 5 at 72,757,469 bp
  • T to A, chromosome 5 at 75,615,491 bp
  • T to C, chromosome 5 at 89,683,184 bp
  • A to G, chromosome 5 at 108,319,939 bp
  • A to G, chromosome 5 at 114,718,417 bp
  • A to G, chromosome 5 at 122,932,919 bp
  • T to A, chromosome 5 at 134,203,334 bp
  • C to A, chromosome 6 at 38,297,947 bp
  • T to G, chromosome 6 at 97,134,330 bp
  • A to G, chromosome 7 at 43,528,838 bp
  • A to T, chromosome 7 at 46,267,342 bp
  • G to A, chromosome 7 at 86,225,750 bp
  • A to G, chromosome 7 at 119,542,393 bp
  • C to T, chromosome 8 at 22,157,190 bp
  • C to A, chromosome 8 at 40,754,759 bp
  • C to T, chromosome 8 at 70,788,252 bp
  • G to A, chromosome 8 at 85,055,206 bp
  • T to A, chromosome 9 at 15,999,109 bp
  • A to C, chromosome 9 at 19,942,079 bp
  • T to A, chromosome 9 at 37,855,296 bp
  • T to C, chromosome 9 at 106,719,641 bp
  • T to C, chromosome 10 at 41,482,724 bp
  • T to A, chromosome 10 at 50,731,648 bp
  • C to A, chromosome 10 at 80,714,301 bp
  • C to A, chromosome 10 at 86,733,714 bp
  • C to T, chromosome 10 at 86,905,539 bp
  • A to G, chromosome 10 at 121,267,543 bp
  • G to T, chromosome 10 at 127,548,346 bp
  • A to G, chromosome 11 at 69,854,984 bp
  • A to T, chromosome 11 at 72,823,416 bp
  • A to G, chromosome 11 at 107,025,927 bp
  • C to T, chromosome 12 at 81,918,819 bp
  • T to G, chromosome 12 at 84,934,958 bp
  • A to T, chromosome 12 at 99,388,902 bp
  • A to C, chromosome 12 at 101,588,063 bp
  • A to G, chromosome 13 at 50,701,094 bp
  • T to A, chromosome 13 at 51,665,628 bp
  • T to C, chromosome 13 at 55,383,756 bp
  • A to G, chromosome 13 at 114,307,815 bp
  • A to T, chromosome 14 at 26,665,699 bp
  • A to T, chromosome 14 at 59,423,384 bp
  • A to T, chromosome 15 at 89,421,426 bp
  • C to T, chromosome 16 at 14,589,246 bp
  • C to T, chromosome 16 at 87,914,222 bp
  • C to A, chromosome 16 at 94,328,510 bp
  • T to A, chromosome 17 at 28,530,905 bp
  • C to T, chromosome 17 at 46,164,269 bp
  • T to A, chromosome 17 at 47,703,114 bp
  • A to G, chromosome 17 at 56,270,089 bp
  • A to G, chromosome 17 at 88,549,507 bp
  • T to A, chromosome 18 at 14,804,524 bp
  • T to C, chromosome 19 at 6,830,409 bp
  • T to A, chromosome 19 at 8,955,509 bp
  • T to C, chromosome 19 at 11,828,892 bp
  • C to T, chromosome 19 at 45,956,741 bp
  • T to A, chromosome 19 at 53,529,498 bp
  • T to A, chromosome 19 at 56,314,053 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8021 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067460-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.