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Strain Name:
C57BL/6J-MtgxR8024Btlr/Mmmh
Stock Number:
067463-MU
Citation ID:
RRID:MMRRC_067463-MU
Other Names:
R8024 (G1)
Major Collection:

Strain Information

Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Tfpi
Name: tissue factor pathway inhibitor
Synonyms: A630013F22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21788
Homologene: 4579
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Rrm1
Name: ribonucleotide reductase M1
Synonyms: RnrM1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20133
Homologene: 806
Exoc4
Name: exocyst complex component 4
Synonyms: Sec8, Sec8l1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20336
Homologene: 40654
Helz
Name: helicase with zinc finger domain
Synonyms: 9630002H22Rik, 9430093I07Rik, 3110078M01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78455
Homologene: 8918
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 9,563,855 bp
  • T to A, chromosome 1 at 66,606,644 bp
  • A to G, chromosome 1 at 92,884,994 bp
  • T to C, chromosome 1 at 163,039,233 bp
  • A to G, chromosome 1 at 166,226,627 bp
  • A to G, chromosome 1 at 173,338,055 bp
  • T to C, chromosome 2 at 4,603,702 bp
  • A to T, chromosome 2 at 84,453,922 bp
  • T to C, chromosome 2 at 89,789,046 bp
  • A to T, chromosome 2 at 101,642,507 bp
  • C to T, chromosome 2 at 110,667,783 bp
  • A to G, chromosome 2 at 118,760,524 bp
  • A to G, chromosome 2 at 122,097,321 bp
  • T to C, chromosome 2 at 165,291,855 bp
  • A to T, chromosome 3 at 57,287,765 bp
  • G to A, chromosome 3 at 64,559,824 bp
  • T to A, chromosome 3 at 69,729,965 bp
  • T to A, chromosome 3 at 86,295,401 bp
  • A to C, chromosome 3 at 88,248,453 bp
  • C to T, chromosome 3 at 93,773,168 bp
  • T to C, chromosome 4 at 88,397,000 bp
  • A to G, chromosome 4 at 135,734,278 bp
  • G to A, chromosome 4 at 135,801,748 bp
  • T to A, chromosome 4 at 135,916,885 bp
  • A to G, chromosome 5 at 24,550,636 bp
  • A to C, chromosome 5 at 121,500,923 bp
  • A to T, chromosome 6 at 23,042,751 bp
  • T to A, chromosome 6 at 33,347,931 bp
  • A to G, chromosome 6 at 41,860,364 bp
  • G to A, chromosome 6 at 129,094,425 bp
  • T to A, chromosome 7 at 74,554,470 bp
  • T to A, chromosome 7 at 87,304,910 bp
  • T to A, chromosome 7 at 102,457,265 bp
  • A to T, chromosome 7 at 105,406,835 bp
  • T to C, chromosome 7 at 107,934,438 bp
  • T to A, chromosome 7 at 108,095,289 bp
  • T to C, chromosome 7 at 139,987,576 bp
  • T to C, chromosome 7 at 141,892,826 bp
  • A to G, chromosome 8 at 69,075,147 bp
  • A to T, chromosome 8 at 69,894,537 bp
  • A to T, chromosome 8 at 117,076,182 bp
  • T to G, chromosome 9 at 18,397,473 bp
  • C to A, chromosome 9 at 38,736,020 bp
  • T to C, chromosome 9 at 108,092,591 bp
  • T to A, chromosome 10 at 24,879,683 bp
  • A to T, chromosome 10 at 28,982,595 bp
  • A to C, chromosome 10 at 28,986,217 bp
  • G to T, chromosome 10 at 86,846,052 bp
  • T to A, chromosome 10 at 88,971,332 bp
  • T to C, chromosome 10 at 105,190,126 bp
  • G to A, chromosome 10 at 127,898,882 bp
  • T to G, chromosome 10 at 129,801,943 bp
  • T to A, chromosome 11 at 5,521,910 bp
  • T to A, chromosome 11 at 107,686,421 bp
  • A to T, chromosome 12 at 49,389,626 bp
  • T to C, chromosome 12 at 87,447,600 bp
  • C to T, chromosome 12 at 112,608,902 bp
  • A to G, chromosome 13 at 27,318,695 bp
  • A to T, chromosome 13 at 64,922,804 bp
  • A to G, chromosome 13 at 74,407,051 bp
  • A to T, chromosome 13 at 77,612,594 bp
  • A to G, chromosome 14 at 52,170,875 bp
  • G to T, chromosome 14 at 70,156,667 bp
  • T to A, chromosome 14 at 86,656,399 bp
  • T to C, chromosome 15 at 31,608,171 bp
  • A to G, chromosome 15 at 64,920,246 bp
  • T to A, chromosome 15 at 98,378,997 bp
  • A to T, chromosome 16 at 44,731,415 bp
  • A to T, chromosome 17 at 3,159,293 bp
  • A to T, chromosome 17 at 26,941,452 bp
  • G to A, chromosome 17 at 33,883,203 bp
  • T to C, chromosome 18 at 65,305,035 bp
  • T to A, chromosome 19 at 8,896,140 bp
  • A to G, chromosome 19 at 12,430,206 bp
  • A to T, chromosome 19 at 20,900,817 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8024 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067463-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.