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Strain Name:
C57BL/6J-MtgxR8038Btlr/Mmmh
Stock Number:
067475-MU
Citation ID:
RRID:MMRRC_067475-MU
Other Names:
R8038 (G1)
Major Collection:

Strain Information

Rtn4rl1
Name: reticulon 4 receptor-like 1
Synonyms: Ngr3, Ngrh2, Ngrl2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237847
Homologene: 18611
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Chuk
Name: conserved helix-loop-helix ubiquitous kinase
Synonyms: Chuk1, IKK1, IKK[a], IKK-alpha, IkappaB kinase alpha, IKK 1, IKK alpha, IKKalpha, IKK-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12675
HGNC: HGNC:1974
Homologene: 979
Scarb2
Name: scavenger receptor class B, member 2
Synonyms: LIMP II, LGP85, Cd36l2, LIMP-2, 9330185J12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12492
HGNC: HGNC:1665
Homologene: 48353
Uba2
Name: ubiquitin-like modifier activating enzyme 2
Synonyms: Sumo-1 activating enzyme subunit 2, UBA2, anthracycline-associated resistance, Arx, SAE2, Uble1b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50995
Homologene: 4018
Llgl2
Name: LLGL2 scribble cell polarity complex component
Synonyms: 9130006H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217325
HGNC: HGNC:6629
Homologene: 3323
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 176,044,897 bp
  • A to T, chromosome 2 at 89,054,868 bp
  • A to T, chromosome 2 at 93,842,917 bp
  • A to T, chromosome 2 at 125,832,286 bp
  • A to G, chromosome 2 at 168,900,012 bp
  • A to T, chromosome 2 at 173,101,905 bp
  • A to T, chromosome 2 at 178,403,778 bp
  • T to A, chromosome 3 at 14,565,830 bp
  • A to T, chromosome 3 at 155,164,001 bp
  • T to G, chromosome 4 at 112,119,806 bp
  • T to C, chromosome 4 at 126,098,097 bp
  • T to C, chromosome 5 at 32,959,348 bp
  • A to T, chromosome 5 at 92,451,448 bp
  • T to C, chromosome 5 at 114,256,597 bp
  • T to C, chromosome 5 at 122,960,895 bp
  • C to T, chromosome 6 at 47,821,254 bp
  • A to G, chromosome 6 at 52,204,329 bp
  • T to A, chromosome 6 at 85,817,685 bp
  • C to G, chromosome 7 at 3,737,320 bp
  • A to G, chromosome 7 at 29,260,129 bp
  • A to T, chromosome 7 at 34,147,597 bp
  • A to G, chromosome 7 at 44,529,886 bp
  • C to T, chromosome 7 at 90,469,622 bp
  • C to A, chromosome 7 at 144,514,916 bp
  • A to T, chromosome 8 at 21,963,439 bp
  • G to T, chromosome 9 at 19,713,680 bp
  • A to C, chromosome 9 at 20,941,564 bp
  • T to A, chromosome 9 at 39,942,881 bp
  • T to C, chromosome 9 at 66,060,333 bp
  • A to G, chromosome 9 at 108,957,292 bp
  • T to C, chromosome 11 at 60,538,985 bp
  • T to C, chromosome 11 at 72,253,370 bp
  • T to C, chromosome 11 at 74,392,283 bp
  • G to C, chromosome 11 at 75,265,881 bp
  • T to A, chromosome 11 at 84,215,904 bp
  • T to G, chromosome 11 at 115,851,103 bp
  • A to G, chromosome 11 at 115,927,352 bp
  • T to C, chromosome 12 at 110,936,420 bp
  • A to T, chromosome 12 at 113,269,433 bp
  • A to G, chromosome 12 at 115,145,590 bp
  • T to C, chromosome 13 at 100,162,062 bp
  • A to T, chromosome 13 at 114,853,755 bp
  • A to G, chromosome 14 at 34,691,682 bp
  • A to G, chromosome 14 at 52,299,964 bp
  • A to T, chromosome 14 at 55,718,930 bp
  • A to G, chromosome 15 at 66,688,875 bp
  • A to G, chromosome 15 at 80,160,270 bp
  • C to T, chromosome 17 at 25,375,891 bp
  • G to A, chromosome 19 at 13,655,355 bp
  • A to G, chromosome 19 at 44,078,977 bp
  • A to G, chromosome 19 at 56,897,888 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8038 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067475-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.