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Strain Name:
C57BL/6J-MtgxR8043Btlr/Mmmh
Stock Number:
067480-MU
Citation ID:
RRID:MMRRC_067480-MU
Other Names:
R8043 (G1)
Major Collection:

Strain Information

Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Cep83
Name: centrosomal protein 83
Synonyms: 2600001G24Rik, 5730513H21Rik, 4921537D05Rik, Ccdc41
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77048
VEGA: 10
Homologene: 9396
Ybx3
Name: Y box protein 3
Synonyms: MSY4, dbpA, oxyR, Dpba, Yb2, MSY3, Csda
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56449
HGNC: HGNC:2428
Homologene: 2708
Rufy3
Name: RUN and FYVE domain containing 3
Synonyms: 2810428M05Rik, 6330416M07Rik, D5Bwg0860e, Rpipx
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52822
Homologene: 87809
Cep170
Name: centrosomal protein 170
Synonyms: 4933426L22Rik, A330004A13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545389
Homologene: 22844
Coro1c
Name: coronin, actin binding protein 1C
Synonyms: coronin 3, CRN2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23790
HGNC: HGNC:2254
Homologene: 56537
Slc30a6
Name: solute carrier family 30 (zinc transporter), member 6
Synonyms: ZnT6, ZnT-6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210148
VEGA: 17
Homologene: 32380
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 21,479,420 bp
  • T to G, chromosome 1 at 139,522,448 bp
  • A to T, chromosome 1 at 165,655,342 bp
  • C to A, chromosome 1 at 172,326,056 bp
  • C to T, chromosome 1 at 176,769,242 bp
  • C to T, chromosome 2 at 52,465,651 bp
  • A to T, chromosome 2 at 112,775,664 bp
  • C to T, chromosome 2 at 112,875,077 bp
  • A to T, chromosome 2 at 143,813,530 bp
  • T to C, chromosome 3 at 89,432,928 bp
  • C to T, chromosome 3 at 98,291,128 bp
  • T to C, chromosome 3 at 158,063,760 bp
  • G to A, chromosome 4 at 21,824,543 bp
  • G to A, chromosome 4 at 133,210,960 bp
  • A to G, chromosome 5 at 84,233,654 bp
  • C to A, chromosome 5 at 88,642,992 bp
  • G to A, chromosome 5 at 89,582,654 bp
  • C to T, chromosome 5 at 113,865,759 bp
  • T to C, chromosome 5 at 120,897,017 bp
  • T to C, chromosome 5 at 124,073,602 bp
  • T to C, chromosome 5 at 146,835,892 bp
  • T to C, chromosome 6 at 131,384,506 bp
  • C to G, chromosome 7 at 3,737,320 bp
  • T to A, chromosome 7 at 20,101,293 bp
  • A to T, chromosome 7 at 104,975,873 bp
  • C to T, chromosome 7 at 122,985,245 bp
  • A to G, chromosome 7 at 127,374,053 bp
  • A to T, chromosome 8 at 23,256,715 bp
  • G to T, chromosome 8 at 128,432,023 bp
  • T to C, chromosome 9 at 37,537,021 bp
  • A to T, chromosome 9 at 57,550,598 bp
  • A to T, chromosome 9 at 105,699,020 bp
  • T to C, chromosome 9 at 109,146,626 bp
  • A to T, chromosome 10 at 80,690,012 bp
  • A to G, chromosome 10 at 81,622,406 bp
  • A to C, chromosome 10 at 94,737,942 bp
  • T to C, chromosome 11 at 53,000,223 bp
  • G to A, chromosome 11 at 55,533,862 bp
  • T to C, chromosome 11 at 121,511,880 bp
  • T to C, chromosome 12 at 16,711,789 bp
  • T to C, chromosome 12 at 69,959,215 bp
  • A to C, chromosome 12 at 82,449,926 bp
  • A to T, chromosome 14 at 50,083,910 bp
  • T to C, chromosome 14 at 59,331,273 bp
  • C to A, chromosome 14 at 103,688,346 bp
  • G to A, chromosome 16 at 44,413,691 bp
  • T to G, chromosome 17 at 74,423,023 bp
  • C to T, chromosome 17 at 88,986,390 bp
  • A to G, chromosome 18 at 36,939,526 bp
  • G to C, chromosome 18 at 61,124,875 bp
  • C to T, chromosome 18 at 65,349,830 bp
  • A to T, chromosome 18 at 65,957,119 bp
  • A to C, chromosome 19 at 12,889,731 bp
  • T to A, chromosome 19 at 48,764,295 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8043 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067480-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.