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Strain Detail Sheet

Published:
Revised: 03/02/2024

Strain Name:
C57BL/6J-MtgxR8046Btlr/Mmmh
Stock Number:
067483-MU
Citation ID:
RRID:MMRRC_067483-MU
Other Names:
R8046 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ahcyl2
Name: S-adenosylhomocysteine hydrolase-like 2
Synonyms: 4631427C17Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74340
Homologene: 69337

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Aasdh
Name: aminoadipate-semialdehyde dehydrogenase
Synonyms: A230062G08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231326
Homologene: 71711

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ppp1r1a
Name: protein phosphatase 1, regulatory inhibitor subunit 1A
Synonyms: I-1, protein phosphatase inhibitor-1, inhibitor-1, 0610038N18Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 58200
VEGA: 15
HGNC: HGNC:9286
Homologene: 4905

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Olfm1
Name: olfactomedin 1
Synonyms: Pancortin 1-4, Noelin 2, Noelin 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56177
Homologene: 8612

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Pml
Name: promyelocytic leukemia
Synonyms: Trim19, 1200009E24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18854
HGNC: HGNC:9113
Homologene: 13245

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 55,346,403 bp
  • A to C, chromosome 1 at 91,435,703 bp
  • A to G, chromosome 1 at 189,258,736 bp
  • A to G, chromosome 2 at 28,229,123 bp
  • T to A, chromosome 2 at 41,269,187 bp
  • T to C, chromosome 2 at 73,618,019 bp
  • T to C, chromosome 2 at 76,779,485 bp
  • T to A, chromosome 2 at 90,138,815 bp
  • G to T, chromosome 2 at 155,210,502 bp
  • G to T, chromosome 2 at 155,277,209 bp
  • A to G, chromosome 3 at 9,699,422 bp
  • A to T, chromosome 3 at 54,194,914 bp
  • T to C, chromosome 3 at 64,707,058 bp
  • A to G, chromosome 3 at 90,389,667 bp
  • G to T, chromosome 4 at 49,681,913 bp
  • A to T, chromosome 4 at 66,266,350 bp
  • A to C, chromosome 4 at 141,220,037 bp
  • T to A, chromosome 5 at 5,249,159 bp
  • G to T, chromosome 5 at 76,896,478 bp
  • T to A, chromosome 5 at 86,528,273 bp
  • G to A, chromosome 5 at 113,551,115 bp
  • T to C, chromosome 6 at 29,878,620 bp
  • T to A, chromosome 6 at 66,715,980 bp
  • C to A, chromosome 6 at 66,731,672 bp
  • A to G, chromosome 6 at 69,764,586 bp
  • T to C, chromosome 6 at 118,408,144 bp
  • A to C, chromosome 6 at 121,222,873 bp
  • A to G, chromosome 6 at 146,426,459 bp
  • T to C, chromosome 7 at 81,308,839 bp
  • A to T, chromosome 7 at 81,317,370 bp
  • T to A, chromosome 7 at 103,552,377 bp
  • C to T, chromosome 8 at 71,408,021 bp
  • A to T, chromosome 8 at 124,028,032 bp
  • A to T, chromosome 9 at 37,897,389 bp
  • A to G, chromosome 9 at 43,792,501 bp
  • A to G, chromosome 9 at 58,246,973 bp
  • A to G, chromosome 9 at 79,706,226 bp
  • C to T, chromosome 9 at 102,723,769 bp
  • T to C, chromosome 10 at 26,887,857 bp
  • A to T, chromosome 10 at 115,179,594 bp
  • A to T, chromosome 11 at 29,758,981 bp
  • A to G, chromosome 11 at 55,536,399 bp
  • A to T, chromosome 11 at 86,784,940 bp
  • A to G, chromosome 11 at 87,146,968 bp
  • G to T, chromosome 11 at 101,124,028 bp
  • G to A, chromosome 11 at 101,274,092 bp
  • A to C, chromosome 11 at 101,525,470 bp
  • T to C, chromosome 12 at 87,919,565 bp
  • C to T, chromosome 12 at 108,681,524 bp
  • T to C, chromosome 13 at 64,174,842 bp
  • T to A, chromosome 13 at 67,748,979 bp
  • T to A, chromosome 13 at 100,222,233 bp
  • A to T, chromosome 13 at 119,374,750 bp
  • A to G, chromosome 14 at 70,778,042 bp
  • C to A, chromosome 15 at 9,139,600 bp
  • T to C, chromosome 15 at 42,496,356 bp
  • A to G, chromosome 15 at 76,714,531 bp
  • T to C, chromosome 15 at 101,572,764 bp
  • T to A, chromosome 15 at 103,537,878 bp
  • A to G, chromosome 16 at 10,395,413 bp
  • A to G, chromosome 16 at 20,506,404 bp
  • T to A, chromosome 16 at 77,109,175 bp
  • G to A, chromosome 17 at 28,550,728 bp
  • A to G, chromosome 17 at 68,840,518 bp
  • C to T, chromosome 19 at 5,760,592 bp
  • T to C, chromosome 19 at 42,309,053 bp
  • C to A, chromosome 19 at 53,817,971 bp
  • T to C, chromosome 19 at 56,320,251 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8046 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
067483-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.