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Strain Name:
C57BL/6J-MtgxR8070Btlr/Mmmh
Stock Number:
067505-MU
Citation ID:
RRID:MMRRC_067505-MU
Other Names:
R8070 (G1)
Major Collection:

Strain Information

Nrp2
Name: neuropilin 2
Synonyms: NP2, Npn-2, Npn2, NP-2, 1110048P06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Atg7
Name: autophagy related 7
Synonyms: 1810013K23Rik, Apg7l
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74244
Homologene: 4662
Ndrg4
Name: N-myc downstream regulated gene 4
Synonyms: D8Bwg1337e, Ndr1-rs, SMAP-8, Ndr4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234593
Homologene: 23228
Ly6a
Name: lymphocyte antigen 6 family member A
Synonyms: Sca-1, TAP, Ly-6A/E, Ly-6E.1, Ly-6A.2, Sca1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110454
Homologene: 113718
Thop1
Name: thimet oligopeptidase 1
Synonyms: EP24.15
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 50492
Homologene: 55726
Arid4b
Name: AT-rich interaction domain 4B
Synonyms: RBBP1L1, SAP180, BRCAA1, Rbp1l1, 6720480E17Rik, 6330417L24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94246
Homologene: 12847
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: GCN1L, G431004K08Rik, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 62,745,408 bp
  • A to G, chromosome 1 at 87,440,907 bp
  • T to C, chromosome 1 at 134,876,069 bp
  • C to A, chromosome 1 at 150,649,992 bp
  • T to G, chromosome 1 at 190,160,910 bp
  • G to T, chromosome 2 at 88,446,659 bp
  • G to A, chromosome 2 at 90,791,565 bp
  • C to A, chromosome 2 at 91,158,014 bp
  • A to C, chromosome 2 at 131,561,582 bp
  • T to A, chromosome 2 at 146,353,279 bp
  • T to C, chromosome 3 at 29,979,838 bp
  • A to G, chromosome 3 at 115,101,955 bp
  • A to G, chromosome 3 at 125,714,644 bp
  • A to G, chromosome 4 at 150,617,375 bp
  • T to C, chromosome 5 at 3,643,540 bp
  • A to G, chromosome 5 at 37,173,287 bp
  • A to G, chromosome 5 at 63,898,167 bp
  • A to G, chromosome 5 at 64,112,082 bp
  • A to G, chromosome 5 at 65,652,398 bp
  • T to A, chromosome 5 at 73,421,463 bp
  • A to T, chromosome 5 at 100,060,982 bp
  • T to A, chromosome 5 at 112,334,930 bp
  • T to A, chromosome 5 at 112,791,120 bp
  • T to A, chromosome 5 at 115,588,998 bp
  • T to C, chromosome 5 at 150,478,007 bp
  • A to G, chromosome 6 at 37,957,726 bp
  • G to A, chromosome 6 at 70,074,105 bp
  • A to G, chromosome 6 at 114,697,080 bp
  • T to C, chromosome 6 at 121,875,879 bp
  • T to C, chromosome 6 at 125,278,470 bp
  • T to C, chromosome 6 at 142,448,504 bp
  • G to A, chromosome 7 at 41,286,463 bp
  • A to G, chromosome 7 at 73,451,758 bp
  • A to G, chromosome 7 at 86,922,487 bp
  • A to T, chromosome 7 at 87,002,128 bp
  • A to T, chromosome 7 at 97,575,648 bp
  • A to G, chromosome 7 at 98,626,715 bp
  • A to G, chromosome 7 at 110,052,807 bp
  • T to C, chromosome 8 at 95,700,128 bp
  • C to T, chromosome 8 at 122,642,981 bp
  • T to A, chromosome 8 at 123,893,621 bp
  • A to G, chromosome 9 at 97,799,470 bp
  • A to G, chromosome 10 at 40,294,230 bp
  • A to T, chromosome 10 at 75,578,899 bp
  • C to T, chromosome 10 at 80,836,121 bp
  • T to C, chromosome 10 at 81,079,486 bp
  • A to G, chromosome 10 at 81,598,642 bp
  • A to T, chromosome 11 at 49,514,114 bp
  • G to A, chromosome 11 at 67,683,434 bp
  • T to C, chromosome 11 at 118,024,671 bp
  • A to T, chromosome 12 at 64,474,608 bp
  • C to T, chromosome 12 at 114,880,036 bp
  • T to A, chromosome 13 at 14,136,259 bp
  • T to C, chromosome 13 at 18,239,624 bp
  • T to A, chromosome 13 at 25,202,197 bp
  • G to T, chromosome 13 at 54,719,793 bp
  • T to A, chromosome 14 at 31,256,686 bp
  • T to C, chromosome 14 at 53,757,792 bp
  • T to C, chromosome 14 at 54,632,573 bp
  • T to C, chromosome 15 at 74,997,600 bp
  • T to A, chromosome 15 at 80,672,080 bp
  • T to C, chromosome 15 at 89,185,373 bp
  • A to G, chromosome 17 at 17,538,638 bp
  • A to T, chromosome 17 at 24,901,104 bp
  • A to G, chromosome 17 at 48,145,510 bp
  • A to G, chromosome 19 at 38,701,839 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8070 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067505-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.