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Strain Name:
C57BL/6J-MtgxR8074Btlr/Mmmh
Stock Number:
067508-MU
Citation ID:
RRID:MMRRC_067508-MU
Other Names:
R8074 (G1)
Major Collection:

Strain Information

Ubn2
Name: ubinuclein 2
Synonyms: 6030408G03Rik, 2900060J04Rik, D130059P03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320538
Homologene: 45564
Rnf145
Name: ring finger protein 145
Synonyms: 3732413I11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74315
Homologene: 14427
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Stam2
Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Synonyms: Hbp, 1200004O12Rik, 5730456G07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56324
Homologene: 68490
Htr1b
Name: 5-hydroxytryptamine (serotonin) receptor 1B
Synonyms: 5-HT1B receptor, 5HT1B receptor, 5-HT1B receptor
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15551
HGNC: HGNC:5287
Homologene: 669
Srpk1
Name: serine/arginine-rich protein specific kinase 1
Synonyms: SR protein-specific kinase 1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20815
Homologene: 110962
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 93,505,561 bp
  • G to T, chromosome 1 at 118,462,483 bp
  • A to T, chromosome 2 at 32,774,123 bp
  • A to T, chromosome 2 at 52,706,426 bp
  • C to G, chromosome 2 at 86,228,129 bp
  • T to A, chromosome 2 at 110,950,232 bp
  • A to G, chromosome 2 at 127,729,865 bp
  • A to G, chromosome 2 at 161,927,661 bp
  • A to C, chromosome 2 at 174,644,735 bp
  • A to G, chromosome 2 at 178,302,796 bp
  • A to T, chromosome 3 at 64,547,643 bp
  • A to T, chromosome 3 at 72,917,198 bp
  • G to A, chromosome 3 at 89,335,326 bp
  • G to A, chromosome 3 at 131,204,305 bp
  • GAA to GA, chromosome 4 at 72,138,979 bp
  • T to C, chromosome 4 at 81,349,087 bp
  • A to T, chromosome 4 at 116,051,434 bp
  • T to A, chromosome 4 at 118,569,625 bp
  • A to T, chromosome 4 at 123,286,715 bp
  • A to G, chromosome 4 at 135,969,801 bp
  • A to T, chromosome 4 at 143,991,854 bp
  • A to G, chromosome 5 at 34,676,138 bp
  • G to T, chromosome 5 at 74,519,596 bp
  • C to A, chromosome 5 at 108,680,575 bp
  • T to A, chromosome 6 at 38,440,540 bp
  • T to C, chromosome 6 at 120,492,908 bp
  • T to A, chromosome 6 at 123,335,945 bp
  • T to A, chromosome 7 at 3,323,521 bp
  • T to C, chromosome 7 at 4,920,206 bp
  • A to T, chromosome 7 at 43,351,790 bp
  • A to G, chromosome 7 at 102,542,623 bp
  • A to T, chromosome 7 at 104,975,727 bp
  • A to T, chromosome 7 at 120,799,098 bp
  • A to T, chromosome 7 at 126,624,937 bp
  • A to G, chromosome 8 at 95,252,173 bp
  • T to G, chromosome 8 at 111,071,598 bp
  • T to C, chromosome 8 at 117,758,701 bp
  • A to T, chromosome 9 at 39,393,946 bp
  • G to A, chromosome 9 at 42,971,103 bp
  • A to G, chromosome 9 at 81,631,529 bp
  • T to C, chromosome 10 at 25,992,952 bp
  • T to C, chromosome 10 at 39,120,259 bp
  • A to G, chromosome 10 at 41,451,232 bp
  • A to G, chromosome 10 at 84,713,659 bp
  • G to T, chromosome 10 at 105,826,080 bp
  • A to C, chromosome 10 at 120,089,416 bp
  • T to A, chromosome 11 at 4,175,435 bp
  • C to A, chromosome 11 at 44,557,436 bp
  • T to A, chromosome 11 at 73,940,387 bp
  • T to A, chromosome 11 at 94,112,051 bp
  • T to G, chromosome 11 at 100,386,287 bp
  • T to G, chromosome 11 at 102,041,601 bp
  • T to A, chromosome 11 at 109,938,494 bp
  • T to C, chromosome 12 at 4,134,420 bp
  • A to G, chromosome 12 at 25,057,716 bp
  • A to T, chromosome 12 at 73,344,884 bp
  • G to A, chromosome 12 at 73,700,267 bp
  • C to T, chromosome 14 at 51,571,623 bp
  • C to T, chromosome 15 at 88,915,375 bp
  • A to G, chromosome 16 at 59,284,186 bp
  • T to G, chromosome 17 at 28,622,016 bp
  • T to A, chromosome 17 at 34,703,981 bp
  • G to A, chromosome 18 at 36,675,011 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8074 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067508-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.